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Phylogenetic analysis and species identification of popular shrimp species in southeast China using the first internally transcribed spacer of ribosomal DNA |
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| Authors: | Zhigang Wu Junli Feng Jishuang Chen Shunhua Xu & Zhiyuan Dai |
| Institution: | College of Food Science and Biotechnology Engineering, Zhejiang Gongshang University, Hangzhou, China; Zhejiang Entry-Exit Inspection and Quarantine Bureau, Hangzhou, China; Institute of Bioengineering, Zhejiang Sci-Tech University, Hangzhou, China |
| Abstract: | The ribosomal DNA internally transcribed spacer 1 (ITS1) was investigated in the search for an appropriate genetic marker that was suitable for phylogenetic study and species identification of eight major exported shrimp species in southeast China. Using the selected primers, the amplified ITS1 sequences exhibited a high degree of length polymorphisms, ranging from 448 bp in Metapenaeopsis dalei to 1491 bp in Macrobrachium nipponense . Many microsatellite loci were found at the 5' end and in the middle region of ITS1, which seemed to be associated with intragenomic sequence variation among samples of the same species. This variation might obscure the phylogenetic relationship between some shrimp populations, but the separation of five Penaeus species was well supported. In combination with polymerase chain reaction-restriction fragment length polymerism methods analysis, ITS1 sequences from shrimp species belonging to different families and genera could also be easily discernable. The results suggested that ITS1 was highly variable among different shrimp groups and could be an appropriate marker for species identification and molecular systematic studies. |
| Keywords: | shrimp ribosomal DNA internally transcribed spacer sequence divergence species identification phylogenetic study |