Refrigerated storage and cryopreservation of sperm of red drum, Sciaenops ocellatus L. |
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| Authors: | W R Wayman T R Tiersch R G Thomas |
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| Institution: | School of Forestry, Wildlife, and Fisheries, Louisiana Agricultural Experiment Station, Louisiana State University Agricultural Centre, Baton Rouge, Louisiana, USA;Louisiana Department of Wildlife and Fisheries, Lyle S. St Amant Marine Biological Laboratory, Grand Terre Island, Louisiana, USA |
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| Abstract: | To aid in artificial spawning of sciaenid fishes, the present authors developed techniques to collect, handle and cryopreserve sperm from red drum, Sciaenops ocellatus L. Sperm were collected by removing and slicing the testis, and adding Hanks' balanced salt solution (HBSS) or NaCl solution (each at 200-400 mOsm kg?1) as an extender. Sperm were activated with 800 mOsm kg?1 artificial sea water (ASW) to characterize motility. Sperm reached maximum motility (highest percentage motility observed for that sample) within 8 ± 1 s (mean ± SD) and remained at maximum motility for 33 ± 4 s. Sperm were exposed to graded osmotic pressures of ASW (8-800 mOsm kg?1) to determine the range of osmolalities that elicited motility. Threshold activation (defined as ～10% motility) occurred at 351 ± 4 mOsm kg?1 and complete activation occurred at 539 ± 2 mOsm kg?1. Sperm stored at 200 mOsm kg?1 retained motility for up to 13 days. Dimethyl sulphoxide (DMSO) was used as a cryoprotectant at concentrations ranging from 7.5% to 15% (v:v) in HBSS (200 mOsm kg?1). There were no significant differences among post-thaw motilities of sperm cryopreserved at any concentration of DMSO. Sperm thawed on the benchtop at 21°C had lower post-thaw motility than did sperm thawed at 10, 20, 30, 40, 50 or 60°C in a water bath. |
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