Iron acquisition mechanisms of Flavobacterium psychrophilum |
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| Authors: | Møller J D Ellis A E Barnes A C Dalsgaard I |
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| Institution: | Fish Disease Laboratory, Danish Institute for Fisheries Research, Frederiksberg, Denmark. jdm@dfu.min.dk |
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| Abstract: | Forty strains of Flavobacterium psychrophilum were tested for the production of siderophores using the universal Chrome Azurol S (CAS) assay. The majority of the strains (85%) were CAS positive (CAS+) and some (15%) were CAS negative (CAS-). The cryptic plasmid pCP1 was carried by all positive strains and was lacking from negative strains. While a weak catechol reaction was detectable in CAS+ culture supernatants, the CAS reaction was, to some extent, heat sensitive, questioning whether the positive reaction was caused only by siderophores. The ability to grow in vitro under iron-restricted conditions did not correlate with the CAS reactivity, as growth of both CAS+ and CAS- strains was similarly impaired under iron restriction induced by 2,2 dipyridyl. Suppressed growth under these conditions was restored by addition of FeCl3, haemoglobin and transferrin for both CAS+ and CAS- strains. |
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| Keywords: | cryptic plasmid Flavobacterium psychrophilum haemoglobin utilization iron-restricted growth siderophore production transferrin utilization |
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