Role of major histocompatibility complex II antigen-presentation pathway genes in orange-spotted grouper infected with Cryptocaryon irritans |
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| Authors: | Jun-Jin Deng Shun Xu Yan-Wei Li Dong-Dong Xu Ze-Quan Mo Jia-Zhou Li Xue-Ming Dan Xiao-Chun Luo |
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| Institution: | 1. School of Biology and Biological Engineering, Guangzhou Higher Education Mega Center, South China University of Technology, Guangzhou, China
Institute of Animal Sciences, Guangdong Academy of Agricultural Sciences, Guangzhou, China;2. School of Biology and Biological Engineering, Guangzhou Higher Education Mega Center, South China University of Technology, Guangzhou, China;3. College of Marine Sciences, South China Agricultural University, Guangzhou, China;4. Institute of Animal Sciences, Guangdong Academy of Agricultural Sciences, Guangzhou, China |
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| Abstract: | Cryptocaryon irritans, a pathogen model for fish mucosal immunity, causes skin mucosal and systematic humoral immune response. Where and how MHC II antigen presentation occurs in fish infected with C. irritans remain unknown. In this study, the full-length cDNA of the grouper cysteine protease CTSS was cloned. The expression distributions of six genes (CTSB, CTSL, CTSS, GILT, MHC IIA and MHC IIB) involved in MHC II antigen presentation pathway were tested. These genes were highly expressed in systematic immune tissues and skin and gill mucosal-associated immune tissues. All six genes were upregulated in skin at most time points. Five genes expected CTSS was upregulated in spleen at most time points. CTSB, CTSL and MHC IIA were upregulated in the gill and head kidney at some time points. These results indicate that the presentation of MHC II antigen intensively occurred in local infected skin and gill. Spleen, not head kidney, had the most extensive systematic antigen presentation. In skin, six genes most likely peaked at day 2, earlier than in spleen (5–7 days), marking an earlier skin antibody peak than any recorded in serum previously. This significant and earlier mucosal antigen presentation indicates that specific immune response occurs in local mucosal tissues. |
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| Keywords: | Cryptocaryon irritans Epinephelus coioides MHC II antigen presentation |
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