Inhibiting fungus on largemouth bass eggs with copper sulfate and its toxicity to fry and juveniles |
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| Authors: | David L Straus Cynthia K Ledbetter David L Heikes |
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| Institution: | 1. Harry K. Dupree-Stuttgart National Aquaculture Research Center, U.S. Department of Agriculture, Agricultural Research Service, Stuttgart, Arkansas;2. Dunn's Fish Farms, Inc, Brinkley, Arkansas |
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| Abstract: | This study determined the effectiveness of copper sulfate (CuSO4) to inhibit fungal growth (caused by Saprolegnia spp.) on largemouth bass (LMB) eggs spawned on/in fiber mats in very high-alkalinity/-hardness waters; experiments also determined the toxicity of CuSO4 to LMB fry and juveniles. An untreated control and three CuSO4 concentrations (10, 20, and 40 mg/L) were tested under a flow-through scenario in the effectiveness experiment. Eggs were treated daily until hatching began. Fungal load at the time of hatch had a total area > 3.0 cm2 in the untreated controls, total area 1.0 to <2.0 cm2 in the 10 and 20 mg/L CuSO4 treatments, and total area > 1.0 cm2 in the 40 mg/L CuSO4 treatments. Fungus samples were identified as Saprolegnia australis. The 24-hr median lethal concentration (LC50) values on the LMB yolk-sac and swim-up fry were 32.0 and 4.6 mg/L CuSO4, respectively; the No Observed Effect Concentrations (NOEC) were 16.0 and 0.125 mg/L CuSO4, respectively. Juvenile LMB were extremely tolerant to CuSO4, and their 24-hr LC50 value was 185.5 mg/L; the NOEC was 64 mg/L. This study indicates that CuSO4 can be an important resource for hatcheries to control egg fungus, especially in high-alkalinity/-hardness waters. |
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| Keywords: | copper sulfate egg fungus largemouth bass Saprolegnia spp toxicity |
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