Cryopreservation of Sperm of Farmed European Eel Anguilla anguilla |
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| Authors: | Tamás Müller Béla Urbányi Balázs Váradi Tibor Binder Péter Horn Miklós Bercsényi Ákos Horváth |
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| Institution: | University of Kaposvár, Faculty of Animal Science, H-7400, Kaposvár, Guba S. u. 40. Hungary;Szent István University, Department of Fish Culture H-2103, Gödöllö, Páter Károly u. I. Hungary;University of Veszprém, Georgikon Faculty of Agriculture. H-8360, Keszthely, Deák F. u. 16. Hungary;University of Kaposvár, Faculty of Animal Science, H-7400, Kaposvár, Guba S. u. 40. Hungary;University of Veszprém, Georgikon Faculty of Agriculture, H-8360, Keszthely, Deák F. u. 16. Hungary;Szent István University, Department of Fish Culture H-2103, Gödöllö, Páter Károly u. I. Hungary |
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| Abstract: | Sexual maturation and sperm release were induced in farmed European eels Anguilla anguilla kept exclusively in fresh water by using two dosages of human chorion gonadotropin (100 International Unit (IU)-Group one and 250 IU/individual per week-Group two). Sperm release took over 13 wk in both groups. The quality of sperm was investigated on the eighth, ninth, and tenth wk. The average cell densities were 0.94 ± 0.4 × 1010 (Group one) and 0.93 to. ± 0.6 × 1010 (Group two) spermatozoa/mL. The estimated motility of eel sperm was 33, 55, and 49% on the eighth, ninth, and tenth wk of treatment, respectively. The estimated average motility of samples selected for cryopreservation was 73 ± 10%, while the post-thaw motility of cryopreserved samples was 36 ± 11%. The extender originally developed for common carp sperm crypreservation together with methanol as cryoprotectant was found suitable for the cryopreservation of European eel sperm. |
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