| 基于基因分型测序(GBS)技术分析鲂鲌鱼类及其杂交子代的遗传结构 |
| |
| 引用本文： | 刘凯,冯晓宇,沈玉帮,马恒甲,李家乐,郭炜,谢楠.基于基因分型测序(GBS)技术分析鲂鲌鱼类及其杂交子代的遗传结构[J].水产学报,2021,45(8):1307-1316. |
| |
| 作者姓名： | 刘凯 冯晓宇 沈玉帮 马恒甲 李家乐 郭炜 谢楠 |
| |
| 作者单位： | 杭州市农业科学研究院,杭州市农业科学研究院,上海海洋大学,杭州市农业科学研究院,上海海洋大学,杭州市农业科学研究院,杭州市农业科学研究院 |
| |
| 基金项目： | 国家大宗淡水鱼产业技术体系杭州综合试验站项目; 杭州市农业科研主动设计项目; 杭州市农科院创新基金项目 |
| |
| 摘 要： | 为利用基因分型测序(GBS)技术对三角鲂、翘嘴鲌、蒙古鲌及其杂交子代的遗传结构进行分析。本研究采用GBS技术对三角鲂、翘嘴鲌、蒙古鲌及其杂交子代(F1)共6个个体的鳍条提取总DNA进行双酶切简化基因组测序,使用Stacks软件构建SNP比对参考基因组进行分析。结果显示,6个个体共产生clean data 7.01 GB,平均每个样品1.17 GB;将所有样本作为一个群体检测SNP变异,共检测出SNP位点399 145个,质控过滤后得到SNP位点97 911个。基于亲本及其子代的SNP位点分析表明,亲本及其子代的平均观测杂合度(H_o)为0.339 4,平均期望杂合度(He)为0.285 3,平均多态性信息含量(PIC)为0.273 7,平均核苷酸多样性(Pi)为0.372 7,平均最小等位基因频率(MAF)为0.247 2,平均可分型样品比例(genotype rate)为93.94%。遗传分化指数和遗传距离分析结果表明,三角鲂、翘嘴鲌、蒙古鲌及其杂交子代间的分化指数为0.309 6～0.894 0,遗传距离为0.370 5～2.244 3。使用最大似然(ML)法构建的系统进化树显示,三角鲂、翘嘴鲌、蒙古鲌及其杂交子代区分明显。本研究通过对三角鲂、翘嘴鲌、蒙古鲌及其杂交子代的遗传结构分析,将为鲂鲌鱼类的杂交选育提供更多的遗传学数据。
|
| 关 键 词： | 三角鲂 翘嘴鲌 蒙古鲌 杂交 单核苷酸多态性 基因结构 基因分型测序 |
| 收稿时间： | 2020/7/3 0:00:00 |
| 修稿时间： | 2020/11/12 0:00:00 |
Genetic structure analysis of Megalobrama terminalis, Culter alburnus, Chanodichthys mongolicus and their hybrids based on genotyping by sequencing |
| |
| LIU Kai,FENG Xiaoyu,SHEN Yubang,MA Hengji,LI Jiale,GUO Wei,XIE Nan.Genetic structure analysis of Megalobrama terminalis, Culter alburnus, Chanodichthys mongolicus and their hybrids based on genotyping by sequencing[J].Journal of Fisheries of China,2021,45(8):1307-1316. |
| |
| Authors: | LIU Kai FENG Xiaoyu SHEN Yubang MA Hengji LI Jiale GUO Wei XIE Nan |
| |
| Institution: | Hangzhou Academy of Agricultural Sciences,Hangzhou Academy of Agricultural Sciences,Shanghai Ocean University,Hangzhou Academy of Agricultural Sciences,Shanghai Ocean University,Hangzhou Academy of Agricultural Sciences,Hangzhou Academy of Agricultural Sciences |
| |
| Abstract: | The aim of this study is to analyze the genetic structure of Megalobrama terminalis, Culter alburnus, Chanodichthys mongolicus and their hybrids by genotyping by sequencing (GBS) technology. In the study, the total DNA extracted from the fin strips of 6 individuals including Megalobrama terminalis, Culter alburnus, Chanodichthys mongolicus and their hybrids (F1), was double digested. Simplified genome sequencing was performed using Stacks software to construct a reference genome for SNP comparison. As a result, 6 individuals produced a total of 7.01 GB of clean data, with an average of 1.17 GB per sample. All samples were used as a group to detect SNP mutations. A total of 399,145 SNP sites were detected. After quality control filtering, 99,911 SNP sites were obtained. SNP analysis based on the parents and their hybrids indicated that the average observed heterozygosity was 0.3394, the average expected heterozygosity was 0.2853, the average polymorphic information content was 0.2737, average nucleotide diversity was 0.3727, the average minimum allele frequency was 0.2472, and the average fractionable sample ratio was 93.94%. Based on the genetic differentiation index and genetic distance between different populations, the results show that the differentiation index between Megalobrama terminalis, Culter alburnus, Chanodichthys mongolicus and their hybrids is 0.3096~0.8940, and the genetic distance is 0.3705~2.2443. The phylogenetic tree constructed using the maximum likelihood (ML) method shows that Megalobrama terminalis, Culter alburnus, Chanodichthys mongolicus and their hybrids are clearly distinguished. This study will provide more genetic data for the hybrid breeding of Megalobrama terminalis, Culter alburnus, and Chanodichthys mongolicus by analyzing the genetic structure. |
| |
| Keywords: | Megalobrama terminalis Culter alburnus Chanodichthys mongolicus Hybrid Single Nucleotide Polymorphism Genetic structure Genotyping by sequencing |
| 本文献已被 CNKI 等数据库收录！ |
| 点击此处可从《水产学报》浏览原始摘要信息 |
| 点击此处可从《水产学报》下载免费的PDF全文 |
|
