| 半滑舌鳎StAR基因克隆及其在不同组织的时空表达分析 |
| |
| 引用本文： | 朱颖,孟亮,胡乔木,王晓夏,常亚青,陈松林.半滑舌鳎StAR基因克隆及其在不同组织的时空表达分析[J].水产学报,2014,38(9):1221-1229. |
| |
| 作者姓名： | 朱颖 孟亮 胡乔木 王晓夏 常亚青 陈松林 |
| |
| 作者单位： | 大连海洋大学水产与生命学院, 辽宁 大连 116023;中国水产科学研究院黄海水产研究所, 农业部海洋渔业可持续发展重点实验室, 山东 青岛 266071;中国水产科学研究院黄海水产研究所, 农业部海洋渔业可持续发展重点实验室, 山东 青岛 266071;中国水产科学研究院黄海水产研究所, 农业部海洋渔业可持续发展重点实验室, 山东 青岛 266071;中国水产科学研究院黄海水产研究所, 农业部海洋渔业可持续发展重点实验室, 山东 青岛 266071;大连海洋大学水产与生命学院, 辽宁 大连 116023;中国水产科学研究院黄海水产研究所, 农业部海洋渔业可持续发展重点实验室, 山东 青岛 266071 |
| |
| 基金项目： | 国家自然科学基金（31130057）；国家“八六三”高技术研究发展计划（2012AA092203）；山东省泰山学者工程专项 |
| |
| 摘 要： | 利用半滑舌鳎性腺转录组测序获得的StAR基因部分序列,设计RACE引物,克隆了半滑舌鳎StAR基因的cDNA序列,全长为1 294 bp,5'端UTR为132 bp,3'端UTR为310 bp,开放阅读框(ORF)为852 bp,共编码283个氨基酸。将半滑舌鳎StAR基因与其他物种StAR基因进行氨基酸同源性分析,结果显示,半滑舌鳎StAR与塞内加尔鳎、大口黑鲈、花鲈、金头鲷的同源性都达到了85%,与虹鳟、斜带石斑鱼及日本鳗鲡的同源性分别为81%、83%和76%。雌、雄鱼不同组织StAR基因的表达分析表明,StAR基因在雄鱼性腺中高表达,在雄鱼的肝脏、脑及心脏中表达量较低,而在雄鱼的其他组织中不表达;在雌鱼肠中不表达,在其他组织(卵巢、肝脏、脾脏、脑、垂体、肌肉、心脏、肾脏)中微量表达。荧光定量PCR分析不同组织与不同时期性腺表达谱表明,雄鱼性腺中StAR基因的表达量显著高于雌、雄鱼其他各组织(P0.05),提示StAR基因对雄鱼精巢发育起重要作用。雄鱼不同时期表达谱分析结果显示,StAR基因在66天前的精巢中不表达,在150天时表达量急剧增加,至2龄时表达量最高,3龄时表达量下降,说明该基因在精巢发育成熟过程中起重要作用。原位杂交结果显示,StAR基因主要在雄鱼精巢的精子细胞中表达,而在雌鱼的卵巢中不表达。研究表明,StAR基因在半滑舌鳎精巢发育中发挥作用,且可能在精子形成中发挥重要作用。
|
| 关 键 词： | 半滑舌鳎 StAR基因 克隆 实时荧光定量PCR 原位杂交 |
| 收稿时间： | 1/1/2014 12:00:00 AM |
| 修稿时间： | 2014/4/18 0:00:00 |
Molecular cloning and temporal expression analysis of StAR gene in different tissues of half-smooth tongue sole(Cynoglossus semilaevis) |
| |
| ZHU Ying,MENG Liang,HU Qiaomu,WANG Xiaoxi,CHANG Yaqing and CHEN Songlin.Molecular cloning and temporal expression analysis of StAR gene in different tissues of half-smooth tongue sole(Cynoglossus semilaevis)[J].Journal of Fisheries of China,2014,38(9):1221-1229. |
| |
| Authors: | ZHU Ying MENG Liang HU Qiaomu WANG Xiaoxi CHANG Yaqing and CHEN Songlin |
| |
| Institution: | College of Fisheries and Life Science, Ocean University of Dalian, Dalian 116023, China;Key Lab for Sustainable Utilization of Marine Fisheries Resources, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China;Key Lab for Sustainable Utilization of Marine Fisheries Resources, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China;Key Lab for Sustainable Utilization of Marine Fisheries Resources, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China;Key Lab for Sustainable Utilization of Marine Fisheries Resources, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China;College of Fisheries and Life Science, Ocean University of Dalian, Dalian 116023, China;Key Lab for Sustainable Utilization of Marine Fisheries Resources, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China |
| |
| Abstract: | Partial StAR gene sequence obtained from whole genome sequencing results of half-smooth tongue sole(Cynoglossus semilaevis)and its full-length cDNA sequence were acquired by SMART-RACE.The StAR complete cDNA was 1 294 bp in length containing 852 bp open reading frame(ORF)and encoded 283 amino acids,132 bp 5'un-translated region(5'-UTR)and 310 bp 3'un-translated region(3'-UTR).Alignment analysis of the amino acids sequence with other species showed that StAR of C.semilaevis was 85% similar to most species of StAR including Solea senegalensis,Micropterus salmoides,Lateolabrax japonicus and Sparus aurata.In order to study the relative expression of StAR gene between females and males,and in different stages of male,we chose the real-time fluorescence quantitative methods.The results indicated that StAR mRNA was extremely highly expressed in testis,but was hardly expressed in the ovary of C.semilaevis.From 16 days to 66 days,StAR gene was not expressed in testis.While 150 days the expression was significantly increased,until sexual maturity,StAR mRNA doubled the amount of expression.After the discharge of sperm,the expression declined obviously.This suggests that the gene has great function in the development of testis.To determine the temporal StAR expression,we chose the method of in situ hybridization.The result showed that StAR mRNA was expressed in sperm cells of testis.Our results showed that StAR gene played important roles in the testis maturation and displayed great function during the period of sperm formation. |
| |
| Keywords: | Cynoglossus semilaevis StAR gene cloning real-time PCR in situ hybridization |
| 本文献已被 CNKI 等数据库收录！ |
| 点击此处可从《水产学报》浏览原始摘要信息 |
| 点击此处可从《水产学报》下载免费的PDF全文 |
|
