| 初探母源免疫处理草鱼母本及其子代5种免疫因子含量的周年变化规律 |
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| 引用本文： | 刘益,丁春华,吕钊,余建波,王红权,张学文,石存斌,任燕,王庆,吕丽刚,刘科均,李东放,肖调义,刘巧林.初探母源免疫处理草鱼母本及其子代5种免疫因子含量的周年变化规律[J].水产学报,2022,46(12):2396-2408. |
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| 作者姓名： | 刘益 丁春华 吕钊 余建波 王红权 张学文 石存斌 任燕 王庆 吕丽刚 刘科均 李东放 肖调义 刘巧林 |
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| 作者单位： | 湖南农业大学,湖南农业大学,湖南农业大学,湖南农业大学,湖南农业大学,湖南农业大学,中国水产科学研究院珠江水产研究所,中国水产科学研究院珠江水产研究所,中国水产科学研究院珠江水产研究所,湖南农业大学,湖南农业大学,湖南农业大学,湖南农业大学,湖南农业大学 |
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| 基金项目： | 现代农业产业技术体系建设专项(CARS-45-48)；湖南省教育厅科学研重点项目(20A243) |
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| 摘 要： | 为探究草鱼母源免疫因子传递特性，本实验借助ELISA和qPCR技术分别检测分析了GCRV弱毒疫苗免疫草鱼母本及其子代（即母源免疫子代）早期胚胎阶段至一龄期间5种免疫因子（IgM、C3、LZM、MBL和Bf）蛋白活性和基因表达水平的周年变化规律。结果显示，草鱼母本经GCRV弱毒疫苗免疫后，血液中5种免疫因子蛋白活性及基因表达水平在产卵期均显著高于对照组1.5倍以上，至免疫后第11个月与对照组无显著差异。母源免疫子代发育至水花（4 d）时，IgM、C3、MBL和Bf的基因表达水平均高于对照组；发育至乌仔（15 d）时，IgM、C3和Bf的蛋白活性水平均略高于对照组；发育至5~11月龄时，5种免疫因子在肝脏、脾脏、肾脏和头肾等免疫器官中仍存在高表达。研究表明，GCRV弱毒疫苗促使母源免疫因子在亲本和子代体内富集表达具有一定的时效性，最长可维持至11个月，为实际生产中草鱼母本疫苗免疫程序的制定提供参考依据。
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| 关 键 词： | 草鱼 草鱼呼肠孤病毒 母源免疫因子 早期发育 时效性 保护效果 |
| 收稿时间： | 2021/4/2 0:00:00 |
| 修稿时间： | 2021/5/17 0:00:00 |
Preliminary study on the annual variation of five immune factors in female parent and offspring of Ctenopharyngodon idella with maternal immunization by grass carp reovirus (GCRV) attenuated vaccine |
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| LIU Yi,DING Chunhu,LV Zhao,YU Jianbo,WANG Hongquan,ZHANG Xuewen,SHI Cunbin,REN Yan,WANG Qing,LV Ligang,LIU Kejun,LI Dongfang,XIAO Tiaoyi and LIU Qiaolin.Preliminary study on the annual variation of five immune factors in female parent and offspring of Ctenopharyngodon idella with maternal immunization by grass carp reovirus (GCRV) attenuated vaccine[J].Journal of Fisheries of China,2022,46(12):2396-2408. |
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| Authors: | LIU Yi DING Chunhu LV Zhao YU Jianbo WANG Hongquan ZHANG Xuewen SHI Cunbin REN Yan WANG Qing LV Ligang LIU Kejun LI Dongfang XIAO Tiaoyi and LIU Qiaolin |
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| Institution: | Hunan Agricultural University,,,,,,,,,,,,,Hunan Agricultural University |
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| Abstract: | Ctenopharyngodon idella (C. idella) is the largest cultured freshwater fish species in China, while the healthy development of grass carp industry is long-term restricted by grass carp hemorrhage disease caused by grass carp reovirus (GCRV) infection. The selective breeding of C. idella with high resistance is a research focus of industry and academia. Our previous research found that maternal immunization could significantly enhance the offspring resistance to GCRV. To investigate the expression characteristics of maternal immune factors in the female parent treated with GCRV attenuated vaccine and its offspring, the protein concentration and gene expression levels of five different immune factors (IgM, C3, LZM, MBL, Bf) were detected by ELISA and qPCR in the blood of female parent, the early development embryos and juvenile fish of offspring. The results showed that the protein concentration and gene expression level of five immune factors in female parent blood by GCRV attenuated vaccine (experimental group) were 1.5 times higher than those in the control group, but there was no significant difference between the two groups after 11 months of immunization. The gene expression level of IgM, C3, MBL and Bf in experimental group offspring were higher than those of the control group on day 4. The protein concentration level of IgM, C3 and Bf in experimental group offspring were slightly higher than those of the control group on day 15. Five immune factors were still highly expressed in experimental group offspring immune organs such as liver, spleen, kidney and head kidney at the age of 5-11 months. The results indicated that the expression levels of immune factors in the female parent treated with GCRV attenuated vaccine and its offspring significantly increased after maternal immunization by GCRV attenuated vaccine, but it has a certain time-effectiveness. The high expression of immune factors in the female parents and offspring might only last within 11 months, which provides a reference for the maternal immune procedure in C. idella and molecular information for the breeding of C. idella with viral resistance. |
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| Keywords: | Ctenopharyngodon idella grass carp reovirus maternal immune factor early development time-effectiveness preventive effect |
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