| 鞍带石斑鱼冷冻精子与云纹石斑鱼杂交家系建立及遗传效应 |
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| 引用本文： | 田永胜,陈张帆,段会敏,马文辉,唐江,李文升,刘江春,侯云霞,孙振翔,庞尊方,王晓梅,翟介明,李波.鞍带石斑鱼冷冻精子与云纹石斑鱼杂交家系建立及遗传效应[J].水产学报,2017,41(12):1817-1828. |
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| 作者姓名： | 田永胜 陈张帆 段会敏 马文辉 唐江 李文升 刘江春 侯云霞 孙振翔 庞尊方 王晓梅 翟介明 李波 |
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| 作者单位： | 1. 中国水产科学研究院黄海水产研究所,农业部海洋渔业可持续发展重点实验室,山东 青岛 266071;青岛海洋科学与技术国家实验室,海洋渔业科学与食物产出过程功能实验室,山东 青岛 266200;2. 中国水产科学研究院黄海水产研究所,农业部海洋渔业可持续发展重点实验室,山东 青岛 266071;上海海洋大学水产与生命学院,上海 201306;3. 莱州明波水产有限公司,山东 莱州,261400 |
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| 基金项目： | 青岛海洋科学与技术国家实验室鳌山科技创新计划（2015ASKJ02）；烟台市科技计划（2016JH021）；烟台市高端人才引进“双百计划” |
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| 摘 要： | 利用4尾鞍带石斑鱼冷冻精子与27尾云纹石斑鱼雌鱼授精建立父系半同胞家系28个,在家系鱼苗生长到130 d时,从所有家系中共选取1294尾鱼苗,对其全长、体长、头长、体高和体质量5个生长性状进行测量。利用单因素方差分析法比较4组父系半同胞家系的生长性能,利用线形混合模型(line mixed mode)对母本和父本遗传效应进行预测,利用最小范数二次无偏估计法(MINQUE)对5个生长性状方差组分进行估计,利用"加性-显性"模型对生长性状遗传相关、遗传力进行估计,采用调整无偏预测法(adjusted unbiased prediction,AUP)预测父母本的加性及显性随机遗传效应。结果显示,5个生长性状之间的加性、显性、基因型和表现型遗传相关基本达到了显著或极显著水平;5个性状的狭义遗传力为(0.5 2 1±0.0 2 0)~(0.8 0 5±0.0 1 8),广义遗传力为(0.5 2 1±0.017)~(0.832±0.011)。27个雌性亲本中有7个(5766、6436、5779、5798、5782、6440、5790)在5个生长性状上达到了极显著的正向加性效应,4个雄性亲本中有1个(8920)达到了极显著的正向加性效应,其后代生长指标显著高于其他雄性的后代。28个杂交组合中有7个(5766*5777、6436*8932、5779*8932、5798*8932、5782*8932、6440*8920、5790*8920)在头长、体高和体质量3个性状上达到了显著或极显著的正向显性效应。本研究首次开展了鞍带石斑鱼冷冻精子与云纹石斑鱼远缘杂交及遗传效应预测研究,为石斑鱼远缘杂交育种技术和新品种培育提供了丰富的科学依据。
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| 关 键 词： | 鞍带石斑鱼 云纹石斑鱼 冷冻精子 远缘杂交 遗传力 遗传效应 |
| 收稿时间： | 2016/12/4 0:00:00 |
| 修稿时间： | 2017/3/19 0:00:00 |
The family line establishment of the hybrid Epinephelus moara (♀) ×E. lanceolatus (♂) by using cryopreserved sperm and the related genetic effect analysis |
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| TIAN Yongsheng,CHEN Zhangfan,DUAN Huimin,MA Wenhui,TANG Jiang,LI Wensheng,LIU Jiangchun,HOU Yunxi,SUN Zhenxiang,PANG Zunfang,WANG Xiaomei,ZHAI Jieming and LI Bo.The family line establishment of the hybrid Epinephelus moara (♀) ×E. lanceolatus (♂) by using cryopreserved sperm and the related genetic effect analysis[J].Journal of Fisheries of China,2017,41(12):1817-1828. |
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| Authors: | TIAN Yongsheng CHEN Zhangfan DUAN Huimin MA Wenhui TANG Jiang LI Wensheng LIU Jiangchun HOU Yunxi SUN Zhenxiang PANG Zunfang WANG Xiaomei ZHAI Jieming and LI Bo |
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| Institution: | Key Laboratory of Sustainable Development of Marine Fisheries, Minstry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China;Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266200, China,Key Laboratory of Sustainable Development of Marine Fisheries, Minstry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China;Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266200, China,Key Laboratory of Sustainable Development of Marine Fisheries, Minstry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China;College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, China,Mingbo Aquatic Co. Ltd., Laizhou 261400, China,Key Laboratory of Sustainable Development of Marine Fisheries, Minstry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China;College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, China,Mingbo Aquatic Co. Ltd., Laizhou 261400, China,Mingbo Aquatic Co. Ltd., Laizhou 261400, China,Mingbo Aquatic Co. Ltd., Laizhou 261400, China,Mingbo Aquatic Co. Ltd., Laizhou 261400, China,Mingbo Aquatic Co. Ltd., Laizhou 261400, China,Mingbo Aquatic Co. Ltd., Laizhou 261400, China,Mingbo Aquatic Co. Ltd., Laizhou 261400, China and Mingbo Aquatic Co. Ltd., Laizhou 261400, China |
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| Abstract: | Hybridization is a way to establish a new gene combination and important genetic progress, through which various desirable traits can be combined to improve varieties. In this study, eggs from 27 females of Epinephelus moara were fertilized with cryopreserved sperm from 4 males of E. lanceolatus, and 28 paternal half-sib families were constructed. At 130d post-hatch, 5 traits including full length, body length, head length, body height and body weight, were measured in 1294 fries from all the families and analyzed by using One-Way ANOVA. Maternal or paternal genetic effects were predicted by using mixed linear model. Variances of five traits were analyzed by using minimum norm quadratic unbiased estimator (MNQUE). Genetic correlation and heritability were analyzed by using "Additive-dominant" genetic model. Maternal and parental additive and dominant random genetic effects were predicted by using adjusted unbiased prediction (AUP). The results showed that significant or extremely significant differences existed in the additive, dominant, genetic and apparent genetic correlations among five traits. Narrow heritability of five traits ranged from 0.521±0.020 to 0.805±0.018, while broad-sense heritability ranged from 0.521±0.017 to 0.832±0.011. Five traits of seven female parents (5766, 6436, 5779, 5798, 5782, 6440, 5790) among 27 gained extremely significant positive additive effect, and one male parent (♂ 8920)among four gained extremely significant positive additive effect. Growth indexes in their offspring were significantly higher than those in other families. Growth indexes of head length, body height and body weight among seven hybrid combinations (5766*5777, 6436*8932, 5779*8932, 5798*8932, 5782*8932, 6440*8920, 5790*8920) among 28 gained significant or extremely significant positive dominant effect. This was the first study to take distant hybridization between E. moara and E. lanceolatus by using cryopreserved sperm and to predict their genetic effects, which provided solid scientific basis for the development of distant hybridization techniques and new variety breeding in groupers. |
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| Keywords: | Epinephelus lanceolatus Epinephelus moara cryopreserved sperm distant hybridization heritability genetic effect |
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