| 棘头梅童鱼七个野生群体遗传多样性的微卫星分析 |
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| 引用本文： | 宋炜,孟永永,蒋科技,赵明,任桂静,张凤英,马凌波.棘头梅童鱼七个野生群体遗传多样性的微卫星分析[J].水产学报,2017,41(1):31-39. |
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| 作者姓名： | 宋炜 孟永永 蒋科技 赵明 任桂静 张凤英 马凌波 |
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| 作者单位： | 农业部东海与远洋渔业资源开发利用重点实验室,农业部东海与远洋渔业资源开发利用重点实验室,农业部东海与远洋渔业资源开发利用重点实验室,农业部东海与远洋渔业资源开发利用重点实验室,农业部东海与远洋渔业资源开发利用重点实验室,农业部东海与远洋渔业资源开发利用重点实验室,农业部东海与远洋渔业资源开发利用重点实验室 |
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| 基金项目： | 中央级公益性科研院所基本科研业务费专项资金(东2015M08)；科技基础性工作专项子课题(2013FY110700)；公益性农业行业专项子课题(201303047) |
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| 摘 要： | 为研究中国沿海地区棘头梅童鱼群体的遗传多样性,利用微卫星标记技术,采用9对微卫星引物对中国连云港(LYG)、大丰(DF)、崇明(CM)、舟山(ZS)、温州(WZ)、宁德(ND)、厦门(XM)棘头梅童鱼7个野生群体的遗传多样性和遗传结构进行了分析。结果显示,实验检测到63个等位基因,各位点等位基因数为3~13个,有效等位基因数为1.7510~8.0317;各位点的观测杂合度(Ho)为0.3596~0.7854,期望杂合度(He)为0.4300~0.8780;多态信息含量(PIC)为0.3604~0.8631,其中有2个位点表现为中度多态(0.25PIC0.5),7个位点表现为高度多态(PIC0.5),具有较丰富的遗传多样性水平。Hardy-Weinber平衡分析显示,7个群体的大部分位点未偏离平衡。基于群体间Nei氏标准遗传距离构建的7个野生群体UPGMA系统进化树结果显示,ND和WZ群体遗传关系最近,ZS和WZ群体遗传关系最远,WZ和ND聚为一支,但总体上没有显著的遗传分化。
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| 关 键 词： | 棘头梅童鱼 野生群体 微卫星 遗传多样性 |
| 收稿时间： | 2016/4/7 0:00:00 |
| 修稿时间： | 2016/7/6 0:00:00 |
Analysis of genetic diversity among seven wild Collichthys lucidus populations by using microsatellite marker |
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| SONG Wei,MENG Yongyong,JIANG Keji,ZHAO Ming,REN Guijing,ZHANG Fengying and MA Lingbo.Analysis of genetic diversity among seven wild Collichthys lucidus populations by using microsatellite marker[J].Journal of Fisheries of China,2017,41(1):31-39. |
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| Authors: | SONG Wei MENG Yongyong JIANG Keji ZHAO Ming REN Guijing ZHANG Fengying and MA Lingbo |
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| Institution: | Key Laboratory of East China Sea and Oceanic Fishery Resources Exploitation,Ministry of Agriculture,East China Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Key Laboratory of East China Sea and Oceanic Fishery Resources Exploitation,Ministry of Agriculture,East China Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Key Laboratory of East China Sea and Oceanic Fishery Resources Exploitation,Ministry of Agriculture,East China Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Key Laboratory of East China Sea and Oceanic Fishery Resources Exploitation,Ministry of Agriculture,East China Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Key Laboratory of East China Sea and Oceanic Fishery Resources Exploitation,Ministry of Agriculture,East China Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Key Laboratory of East China Sea and Oceanic Fishery Resources Exploitation,Ministry of Agriculture,East China Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Key Laboratory of East China Sea and Oceanic Fishery Resources Exploitation,Ministry of Agriculture,East China Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences |
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| Abstract: | In order to study the genetic diversity of Collichthys lucidus of Chinese coastal areas, microsatellite markers were used to analyze genetic diversity of seven wild C. lucidus populations (Lianyungang, Dafeng, Chongming, Zhoushan, Wenzhou, Ningde, Xiamen). The results showed that the number of total alleles was 63 in the seven populations by nine microsatellite loci, and the mean number of alleles of every locus was 3 to 13. The average effect alleles ranged from 1.7510 to 8.0317. The average observed heterozygosity (Ho) ranged from 0.3596 to 0.7854, and the average expected heterozygosity (He) ranged from 0.4300 to 0.8780, and the average polymorphic information content (PIC) ranged from 0.3604-0.8631. Among them, 2 loci showed moderate polymorphic, 7 loci showed highly polymorphic. This showed that seven populations had high levels of genetic diversity. Most loci did not deviate from Hardy-Weinberg equilibrium (P>0.05). The UPGMA phylogenetic tree of the seven populations was constructed based on Nei''s genetic distance. The result showed that the relationship between WZ and ND was the closest while the relationship between ZS and WZ was the farthest, WZ and ND clustered into one group, while there was no significant genetic differentiation as a whole. |
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| Keywords: | Collichthys lucidus wild populations microsatellites genetic diversity |
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