| 牙鲆的淋巴细胞表面抗原 |
| |
| 引用本文： | 乌日琴.牙鲆的淋巴细胞表面抗原[J].水产学报,2003,27(5):486-490. |
| |
| 作者姓名： | 乌日琴 |
| |
| 作者单位： | 1. 中国科学院海洋研究所实验海洋生物学开放实验室,山东,青岛,266071;中国科学院研究生院,北京,100039
2. 中国科学院海洋研究所实验海洋生物学开放实验室,山东,青岛,266071 |
| |
| 基金项目： | 国家"973"项目资助(G1999012003,G1999012006) |
| |
| 摘 要： | 关于鱼类淋巴细胞的异质性的研究开始于20世纪70年代,80年代以后才利用抗IgM单克隆抗体证明硬骨鱼具有T/B样淋巴细胞1-5]。鱼类的T淋巴细胞具有和哺乳动物T细胞相似的功能,如:对同种移植产生的排斥反应和细胞增殖反应。虽然鱼类的T样淋巴细胞还没有象哺乳动物那样建立起以表面多肽的单克隆抗体进行分离鉴定的CD系统,但在硬骨鱼中已发现了编码TCR分子和MHC分子的基因,随着DNA测序技术的迅速发展,美国、法国和日本等国家的几个实验室6-10]在20世纪90年代开展了鱼类TCR基因多样性和组织相容性抗原(MHC)在脊椎动物系统发育中进化关…
|
| 关 键 词： | 淋巴细胞 表面抗原 绵羊红细胞受体 NK细胞 T细胞 牙鲆 |
| 文章编号： | 1000-0615(2003)05-0486-05 |
| 收稿时间： | 2014/3/17 0:00:00 |
| 修稿时间： | 2002年9月3日 |
Study on surface antigen of lymphocyte of Paralichthys oliveaceus |
| |
| WU Ri-qin.Study on surface antigen of lymphocyte of Paralichthys oliveaceus[J].Journal of Fisheries of China,2003,27(5):486-490. |
| |
| Authors: | WU Ri-qin |
| |
| Abstract: | To observe the surface microstructure features by using scanning electron microscope,and study the surface antigen on lymphocytes of Paralichthys oliveaceus by form rosette with sheep red bloods cells and the cross-reactivity by using monoclonal antibody against human CD.Under scanning microscope ,there are two kinds of lymphocytes,type I lymphocytes is characterized with being full of bumpiness and holes, the surface of type II lymphocytes is rather smooth with tiny finger like tubercle. The rosette formation of lymphocytes with sheep red blood cells is obvious however the rosette forming percents of lymphocytes is rather low,lower than 2%. The cross reactivity of lymphocytes with monoclonal antibody against human CD showed that there are 5.25%+0.38% of NK cells in peripherial lymphocytes of Paralichthys oliveaceus,and the CD25~+ percentage of lymphocytes is 5.25%+0.38%.No cross-reactivity with T-cell antigen,B-cell antigen and other activity antigen is found on the lymphocytes surface of Paralichthys oliveaceus. |
| |
| Keywords: | Paralichthys oliveaceus lymphocytes surface antigen sheep red blood cells receptor NK cell T cell |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
| 点击此处可从《水产学报》浏览原始摘要信息 |
| 点击此处可从《水产学报》下载免费的PDF全文 |
|
