| 吉富罗非鱼与奥利亚罗非鱼自繁与杂交 F1遗传特性与抗病力分析 |
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| 引用本文： | 强俊,杨弘,马昕羽,王辉,徐跑,何杰,朱志祥,李瑞伟.吉富罗非鱼与奥利亚罗非鱼自繁与杂交 F1遗传特性与抗病力分析[J].水产学报,2015,39(1):32-41. |
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| 作者姓名： | 强俊 杨弘 马昕羽 王辉 徐跑 何杰 朱志祥 李瑞伟 |
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| 作者单位： | 中国水产科学研究院淡水渔业研究中心, 农业部淡水渔业和种质资源利用重点实验室, 江苏 无锡 214081;中国水产科学研究院淡水渔业研究中心, 农业部淡水渔业和种质资源利用重点实验室, 江苏 无锡 214081;南京农业大学无锡渔业学院, 江苏 无锡 2014081;南京农业大学无锡渔业学院, 江苏 无锡 2014081;淮阴师范学院生命科学学院, 江苏 淮安 223300;中国水产科学研究院淡水渔业研究中心, 农业部淡水渔业和种质资源利用重点实验室, 江苏 无锡 214081;南京农业大学无锡渔业学院, 江苏 无锡 2014081;中国水产科学研究院淡水渔业研究中心, 农业部淡水渔业和种质资源利用重点实验室, 江苏 无锡 214081;中国水产科学研究院淡水渔业研究中心, 农业部淡水渔业和种质资源利用重点实验室, 江苏 无锡 214081;茂名市茂南三高渔业发展有限公司, 广东 茂名 514025 |
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| 基金项目： | "十二五"国家科技支撑计划(2012BAD26B03-1);现代农业产业技术体系专项(CARS-49) |
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| 摘 要： | 以吉富罗非鱼与奥利亚罗非鱼为繁育亲本,采用完全双列杂交繁育4组F1,将初始规格基本一致的4组罗非鱼饲养100 d后,运用“加性-显性”遗传分析模型,分析了4组F1罗非鱼8个生长相关性状杂种优势、遗传效应以及与性状间的相关性.结果表明:(1)F1群体平均优势为0.129 4～0.368 4.除尾柄长超亲优势较大外,其他性状的群体超亲优势较小或表现出负向超亲优势.(2)8个性状的广义遗传率(HB)为0.714 2～0.995 3,表明加性效应和显性效应对性状的遗传变异影响极显著(P＜0.01).除尾柄长外,其他性状的狭义遗传率(HN)介于0.469 4～0.737 9,表明加性遗传方差在表型方差中所占比率较高.(3)体质量、全长、体长、体高、体宽、头长、尾柄长、尾柄高性状之间表型相关在0.776 6～0.999 7范围内,而遗传相关在0.994 1～1.000 0之间,表明这些性状间都存在极显著的正相关.取样结束后,采用3.95×106 CFU/mL的海豚链球菌菌液进行腹腔感染,吉富罗非鱼自繁组F1代12 h后出现死亡,而奥利亚罗非鱼自繁组F1192 h后才出现死亡.384 h后,吉富罗非鱼自繁组F1死亡率为40％,正反交组F1分别为20％和23.3％,奥利亚罗非鱼自繁组F1死亡率最低,为6.67％.研究结果表明,除尾柄长外,杂交F1的其他性状不具备超亲优势,然而杂交可以提高选育后代的抗病力.
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| 关 键 词： | 吉富罗非鱼 奥利亚罗非鱼 遗传特性 杂交优势 海豚链球菌 |
| 收稿时间： | 8/3/2014 12:00:00 AM |
| 修稿时间： | 2014/11/4 0:00:00 |
Comparison of genetic traits and disease resistance of GIFT Oreochromis niloticus,O.aureus and their reciprocal hybrids F1 offspring |
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| QIANG Jun,YANG Hong,MA Xinyu,WANG Hui,XU Pao,HE Jie,ZHU Zhixiang and LI Ruiwei.Comparison of genetic traits and disease resistance of GIFT Oreochromis niloticus,O.aureus and their reciprocal hybrids F1 offspring[J].Journal of Fisheries of China,2015,39(1):32-41. |
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| Authors: | QIANG Jun YANG Hong MA Xinyu WANG Hui XU Pao HE Jie ZHU Zhixiang and LI Ruiwei |
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| Institution: | Freshwater Fisheries Research Center of Chinese Academy of Fishery Sciences, Wuxi 214081, China;Freshwater Fisheries Research Center of Chinese Academy of Fishery Sciences, Wuxi 214081, China;Wuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, China;Wuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, China;School of Life Science, Huaiyin Normal University, Huaian 223300, China;Freshwater Fisheries Research Center of Chinese Academy of Fishery Sciences, Wuxi 214081, China;Wuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, China;Freshwater Fisheries Research Center of Chinese Academy of Fishery Sciences, Wuxi 214081, China;Freshwater Fisheries Research Center of Chinese Academy of Fishery Sciences, Wuxi 214081, China;Maonan Sango Tilapia Breeding Base, Maoming 525024, China |
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| Abstract: | Hybridization(interspecific hybrid)has been used in numerous species to increase growth rate,manipulate sex ratios,produce sterile animals,improve flesh quality,increase disease resistance,improve environmental tolerance and improve a variety of other desirable traits to make aquaculture more profitable.GIFT(Genetically Improved Farmed Tilapia)strain Nile tilapia is the main kind of cultured tilapia,which has many advantages such as rapid growth rate and high fillet yield.However,GIFT tilapia has poor disease resistance capability to Streptococcus iniae infection,which may be related to too much inbreeding.Breed improvement by interspecific hybrid may enhance disease resistance capability in tilapia.A complete diallel mating system between GIFT tilapia and O.aureus and additive-dominant genetic model were used to analyze heterosis of 8 growth-related traits,genetic effect and correlation of traits.At the time of day 100 over the experiment,the results indicated that:(1)Heterosis of growth related traits in F1 hybrid varied from 0.129 4 to 0.368 4,and population heterosis over parent mean(Hpm)of these growths related traits were all positive in F1.In addition to caudal peduncle length(CPL),other growth related traits have negative or insignificant positive population heterosis over parent better(Hpb);(2)The general heritability in the broad sense(HB)with 0.714 2-0.995 3 indicated that genetic variations of all 8 traits were significantly influenced by additive effect and dominance effect(P<0.01),whereas,the narrow sense of growth-related traits except CPL varied from 0.469 4 to 0.737 9,and indicated additive effect controlled the genetic variation of output value;(3)The phenotypic correlations 0.776 6-0.999 7 and genetic correlations 0.994 1-1.000 0 between body weight,body length,total length,body width,body height,head length,caudal peduncle length and caudal peduncle height,were all highly statistically significant(P<0.01).Furthermore,after sampling,experimental fish were infected with 3.95×106 CFU/mL S.iniae through abdominal cavity.F1 generation of GIFT tilapia purebred combination died 12 h later,whereas F1 generation of Aureus tilapia purebred combination died 192 h later.384 h after infection,mortality of F1 for GIFT purebred combination was 40%,while mortality of the reciprocal cross combinations was 20% and 23.3% respectively.The lowest mortality(6.67%)was found in F1 for Aureus purebred combination.Overall,the results indicate that,except CPL,other growth related traits have no heterosis; however,hybridization can improve the disease resistance of breeding offspring. |
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| Keywords: | GIFT Oreochromis niloticus Oreochromis aureus genetic traits heterosis Streptococcus iniae |
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