| 超低温冷冻对西伯利亚鲟精子形态结构损伤的观察 |
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| 引用本文： | 章龙珍.超低温冷冻对西伯利亚鲟精子形态结构损伤的观察[J].水产学报,2008,32(4):558-565. |
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| 作者姓名： | 章龙珍 |
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| 作者单位： | 1. 中国水产科学研究院东海水产研究所农业部海洋与河口渔业重点开放实验室,上海,200090
2. 中国水产科学研究院东海水产研究所农业部海洋与河口渔业重点开放实验室,上海,200090;上海海洋大学上海高校水产养殖E-研究院,上海200090 |
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| 基金项目： | 国家"八六三"高技术研究发展计划
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浙江省科技计划
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国家科技基础条件平台
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上海高校水产养殖E-研究院建设项目
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农业部海洋与河口渔业重点开放实验室开放课题
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上海市科委基础重大专项 |
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| 摘 要： | 为改进西伯利亚鲟精子超低温冷冻保存技术,探讨精子损伤机制,应用扫描和透射电子显微镜,观察了西伯利亚鲟鲜精与冷冻后精子的形态结构.结果显示,经过冷冻保存后精子的形态发生了很大变化.精子顶体长、精子头中部宽、头中部宽与前部宽比值及中段宽与鲜精相比显著增加(P<0.05);中段长度、后外侧延伸物长度比鲜精显著变短(P<0.05).精子经过冷冻后有30.5%的精子在形态、结构上受到不同程度损伤,受损精子显微结构表现为顶体后外侧延伸物与核糅合,顶体内容物丢失;核膜囊泡化、核膜断裂,核内出现空泡;线粒体内嵴弥散,线粒体脱落;鞭毛外膜松弛与鞭毛脱离等.部分受损伤精子出现顶体丢失,中段脱落,鞭毛自中段基部断裂的现象.精子损伤主要集中在膜系统,中心粒等微管系统基本完好.
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| 关 键 词： | 西伯利亚鲟 精子超微结构 冷冻损伤 |
| 收稿时间： | 2007/8/15 0:00:00 |
| 修稿时间： | 1/9/2008 12:00:00 AM |
Observing on cryodamage of spermatozoa of Siberian sturgeon (Acipenser b. baerii) |
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| ZHANG Longzhen.Observing on cryodamage of spermatozoa of Siberian sturgeon (Acipenser b. baerii)[J].Journal of Fisheries of China,2008,32(4):558-565. |
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| Authors: | ZHANG Longzhen |
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| Institution: | East China Sea Fisheries Research Institute |
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| Abstract: | The variation of morphology and ultrastructure between fresh Siberian sturgeon sperm and frozen-thawed sperm were observed by transmission and scanning electron microscopy in this paper. The results showed that the sperm morphology and ultrastructure changed greatly after frozen-thawed. The length of acrosome, the ratio of middle head width to anterior head width and the width of midpiece increased significantly (P<0.05). The length of midpiece and posterolateral projections became short. there was significant difference between fresh and thawed sperm (P<0.05). About 30.5% of the sperms were damaged after frozen-thawed.
The ultrastructure exhibition of freezing injure focused on: the posterolateral projections mixed with nuclear and the content of acrosome lost; the arrangement between acrosomal vesicle, subacrosome and nucleus became slack; the plasma membrane of sperm swelled, folded and disrupted; the membrance of endonuclear canals unclear; the membrane of mitochondria was cracked, dispersed and even mitochondria lost; the membrane of flagellum changed slack and released from flagellum. Some of the damage sperms lost the acrosome, midpiece and flagellum. The flagellum regularly disrupted from the posterior part of midpiece. Most damaged parts focused on the membrane-system of sperm, microtubular-system was still well. Freezing and thawing procedure were the key of cryopreservation. Study of Siberian sturgeon sperm ultrastructure would direct us choose the proper cryopreservant, extender and right method, including freezing and thawing procedure, to conserve the sperm. |
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| Keywords: | Acipenser b baerii sperm ultrastructure damage of cryopreservation |
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