| 间接红细胞凝集反应检测中华鳖血清抗体的方法 |
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| 引用本文： | 杨先乐.间接红细胞凝集反应检测中华鳖血清抗体的方法[J].水产学报,1999,23(1):53-60. |
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| 作者姓名： | 杨先乐 |
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| 作者单位： | 农业部水产增养殖生态、生理重点开放实验室，中国水产科学研究院长江水产研究所 |
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| 基金项目： | 国家自然科学基金,农业部“九五”重点科研项目 |
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| 摘 要： | 建立了间接红细胞凝集反应检测中华鳖血清抗体的方法。试验证明，致敏抗原菌体浓度为３×１０８ｃｆｕ／ｍＬ、ＢＳＡ浓度为２．０ｍｇ／ｍＬ，致敏红细胞浓度２．５％时（无论是人还是绵羊红细胞），本方法能获得最敏感的结果。加入稳定剂后的致敏红细胞，在８０ｄ内检测结果没有显著的差异。本试验测得该方法的灵敏度为１３．４±４．２，而且证明本方法具有较高的特异性、灵敏度、准确性和重复性。
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| 关 键 词： | 中华鳖 抗体 间接 红细胞凝集反应 |
| 收稿时间： | 2014/4/18 0:00:00 |
| 修稿时间： | 2014/4/18 0:00:00 |
A METHOD OF DETECTION OF ANTIBODY IN TRIONY X SINENSIS BY INDIRECT AGGLUTINATION OF RED CELL |
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| YANG XianLe.A METHOD OF DETECTION OF ANTIBODY IN TRIONY X SINENSIS BY INDIRECT AGGLUTINATION OF RED CELL[J].Journal of Fisheries of China,1999,23(1):53-60. |
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| Authors: | YANG XianLe |
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| Institution: | Key Lab of Ecology and Physiology in Aquaculture of the Ministry of Agriculture, Shanghai Fisheries University, 200090 |
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| Abstract: | A method of indirect agglutination of red cell was established, by which antibody in
softshelled turtle, Trionyx sinensis , was detected. It demonstrated that the highest titer could
be obtained while the concentration of sensitizing antigen was 3108cfu/mL for bacteria and
2.0mg/mL for BSA, and that of sensitizing red cell of man or sheep was 2.5%.There was no
significant differences within 80d in the results of detecting with sensitive red cell, to which
stabilizer was added. Furthermore, the sensitivity of this method was higher, and the method
also better in accuracy, specificity and duplicity. |
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| Keywords: | Trionyx sinensis Antibody Indirect agglutination of red cell Detection |
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