| 魁蚶各组织溶菌酶活性对鳗弧菌侵染的响应 |
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| 引用本文： | 赵丹,周丽青,吴彪,孙秀俊,赵峰,杨爱国,刘志鸿,赵庆,张高伟,陈夕.魁蚶各组织溶菌酶活性对鳗弧菌侵染的响应[J].水产学报,2020,44(3):480-486. |
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| 作者姓名： | 赵丹 周丽青 吴彪 孙秀俊 赵峰 杨爱国 刘志鸿 赵庆 张高伟 陈夕 |
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| 作者单位： | 中国水产科学研究院黄海水产研究所,农业农村部海洋渔业可持续发展重点实验室,山东青岛 266071;上海海洋大学水产与生命学院,上海 201306;中国水产科学研究院黄海水产研究所,农业农村部海洋渔业可持续发展重点实验室,山东青岛 266071;青岛海洋科学与技术国家实验室海洋渔业科学与食物产出过程功能实验室,山东青岛 266071;农业农村部东海与远洋渔业资源开发利用重点实验室,上海 200090 |
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| 基金项目： | 农业农村部东海与远洋渔业资源开发利用重点实验室开放课题（2015K02）；山东省重点研发计划（2018GHY115030）；“一带一路”沿线热带国家水产养殖科技创新合作项目 |
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| 摘 要： | 为了有效防治细菌及其他病原微生物对魁蚶等贝类的危害,本实验研究了魁蚶各组织中溶菌酶活性对鳗弧菌侵染的响应过程,以期探讨魁蚶体内溶菌酶的免疫功能。本实验采用注射活菌的方法侵染20月龄魁蚶个体,随机选取16只个体,在每只个体的斧足处注射1 mL(约1×10~9个)鳗弧菌菌悬液作为感染组;随机选取16只个体不注射鳗弧菌作为对照组。两组魁蚶分别于洁净海水中暂养4、12、24和48 h后,每组随机取4只魁蚶个体的血液、外套膜、鳃、斧足、肝胰腺和闭壳肌等组织,采用ELISA试剂盒测定其溶菌酶含量变化。结果显示,对于鳗弧菌的侵入,魁蚶血液中溶菌酶含量由正常低值迅速增高并一直维持较高的含量,说明血液是魁蚶机体防御病原菌的主要免疫组织之一;魁蚶外套膜在无感染的情况下,对外界水环境的干扰始终保持较高的溶菌酶含量;鳃、斧足的溶菌酶含量均在注射细菌24 h之后明显高于正常值,说明外套膜、鳃、斧足作为魁蚶机体与外界接触的第一道屏障也能应对病原菌入侵,但反应较血液延迟;肝胰腺和闭壳肌的溶菌酶含量变化不明显,推测肝胰腺和闭壳肌不是魁蚶的重要免疫组织或器官。本实验结果可为魁蚶抗病选育及免疫机理方面的研究提供相关的参数。
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| 关 键 词： | 魁蚶 溶菌酶 鳗弧菌侵染 |
| 收稿时间： | 2018/12/11 0:00:00 |
| 修稿时间： | 2019/4/25 0:00:00 |
Response of lysozyme activity to Vibrio anguillarum infection in different tissues of Scapharca broughtonii |
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| ZHAO Dan,ZHOU Liqing,WU Biao,SUN Xiujun,ZHAO Feng,YANG Aiguo,LIU Zhihong,ZHAO Qing,ZHANG Gaowei and CHEN Xi.Response of lysozyme activity to Vibrio anguillarum infection in different tissues of Scapharca broughtonii[J].Journal of Fisheries of China,2020,44(3):480-486. |
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| Authors: | ZHAO Dan ZHOU Liqing WU Biao SUN Xiujun ZHAO Feng YANG Aiguo LIU Zhihong ZHAO Qing ZHANG Gaowei and CHEN Xi |
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| Institution: | Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture and Rural Affairs, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China;College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, China,Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture and Rural Affairs, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China;Qingdao National Laboratory of Marine Science and Technology Marine Fishery Science and Food Production Process Function Laboratory, Qingdao 266071, China,Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture and Rural Affairs, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China;Qingdao National Laboratory of Marine Science and Technology Marine Fishery Science and Food Production Process Function Laboratory, Qingdao 266071, China,Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture and Rural Affairs, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China;Qingdao National Laboratory of Marine Science and Technology Marine Fishery Science and Food Production Process Function Laboratory, Qingdao 266071, China,Key Laboratory of Exploitation and Utilization of East China Sea and Ocean Fishery Resources, Ministry of Argriculture and Rual Affairs, Shanghai 200090, China,Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture and Rural Affairs, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China;Qingdao National Laboratory of Marine Science and Technology Marine Fishery Science and Food Production Process Function Laboratory, Qingdao 266071, China,Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture and Rural Affairs, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China;Qingdao National Laboratory of Marine Science and Technology Marine Fishery Science and Food Production Process Function Laboratory, Qingdao 266071, China,Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture and Rural Affairs, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China;College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, China,Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture and Rural Affairs, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China;College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, China and Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture and Rural Affairs, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China;College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, China |
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| Abstract: | In order to effectively prevent the harm of bacteria and other pathogenic microorganisms to S. broughtonii, we observed the response process of the lysozyme activity in different tissues of S. broughtonii infected by Vibrio anguillarum, and explored the immune function of the lysozyme in the body of S. broughtonii. In this experiment, 20-month-old S. broughtonii individuals were infected by injecting live bacteria, and 16 individuals were randomly selected. And 1 mL (about 1×109 bacteria) V. anguillarum suspension was injected into the axe foot of each individual as the infection group. 16 randomly selected individuals were not injected with V. anguillarum as the control group. The two groups were cultivated in clean seawater for 4, 12, 24 and 48 h. In each group, randomly selected 4 S. broughtonii individuals were dissected for the blood, mantle, gill, axe foot, hepatopancrea and adductor muscle tissues. The concentration of lysozyme was determined by ELISA kit. The results showed that for the invasion of V. anguillarum, the lysozyme content in the blood of S. broughtonii rapidly increased from the normal low value and maintained a high level, indicating that the blood is one of the main immune tissues of S. broughtonii body defending against pathogen. In the case of no infection, S. broughtonii mantle always maintained a high lysozyme concentration to prevent the interference of the external water environment. The concentration of lysozyme in infected gills and axe foot are significantly higher than the normal values after the injection for 24 hours, indicating that the mantle, gills and axe foot were the first barriers for S. broughtonii body to contact with the outside world which can also respond to the invasion of pathogenic bacteria, but the response was delayed compared with the blood. The lysozyme content of hepatopancrea and adductor muscle did not change significantly. And it was speculated that hepatopancrea and adductor muscle were not important immune tissues or organs of S. broughtonii. The results of this study can provide relevant parameters for the study of disease resistance and immune mechanism of S. broughtonii. |
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| Keywords: | Scapharca broughtonii lysozyme Vibrio anguillaris infection |
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