| Cu2+胁迫对魁蚶生理生化和组织结构的影响 |
| |
| 引用本文： | 李阳,薛素燕,李加琦,沈淑芳,陈琼琳,蒋增杰,方建光,毛玉泽.Cu2+胁迫对魁蚶生理生化和组织结构的影响[J].水产学报,2018,42(10):1531-1540. |
| |
| 作者姓名： | 李阳 薛素燕 李加琦 沈淑芳 陈琼琳 蒋增杰 方建光 毛玉泽 |
| |
| 作者单位： | 中国水产科学研究院黄海水产研究所农业部海洋渔业可持续发展重点实验室山东省渔业资源与生态环境重点实验室;上海海洋大学水产与生命学院;青岛海洋科学与技术国家实验室海洋生态与环境科学功能实验室 |
| |
| 基金项目： | 国家基金委-山东省联合基金（U1606404）；中国水产科学研究院黄海水产研究所基本科研业务费专项（20603022017002，20603022017010）；国家自然科学基金（41761134052）；国家贝类产业技术体系专项（CARS-49） |
| |
| 摘 要： | 采用生物毒性测试的方法,研究了持续暴露96 h不同浓度Cu2+胁迫对魁蚶生理代谢、组织结构及酶活性的影响。实验设置0.01、0.05、0.10、0.50和1.00 mg/L组5个浓度梯度,不加Cu2+的正常海水作为对照。结果显示,Cu2+胁迫对魁蚶耗氧率(OR)、排氨率(NR)、氧氮比(O∶N)均有显著影响,并且与暴露时间和浓度有关。初次暴露时,OR,NR和O∶N都迅速下降,最低值都出现在1.00 mg/L浓度组,分别为(0.005±0.001)mg/(g·h)、(0.5±0.05)μmol/(g·h)、0.7±0.1,仅为对照组的1%、29%和3%。暴露72 h内Cu2+胁迫均使魁蚶代谢率不同程度下降,之后不同处理组出现差异分化。暴露96 h时,魁蚶对低浓度Cu2+暴露表现出适应,0.01 mg/L处理组呼吸代谢恢复至正常水平,O∶N与对照组无差异,组织结构也未见明显损伤;浓度超过0.05 mg/L处理组魁蚶生理代谢及组织结构受显著影响,O∶N大多降至9以下,出现鳃丝受损和组织结构散乱等明显损伤;0.10 mg/L处理组魁蚶体内ACP和ALP活性在鳃组织中增强,肝脏中受到抑制,而GPX和GST活性增强。研究表明持续暴露96 h Cu2+浓度≥0.05 mg/L环境显著影响魁蚶生理代谢及组织结构,0.10 mg/L Cu2+浓度显著影响魁蚶组织中ACP、ALP、GPX和GST的活性。研究结果为认知魁蚶等滩涂贝类对Cu2+胁迫的响应机制提供基础数据,为预防滩涂潜在重金属污染风险及生物修复提供参考。
|
| 关 键 词： | 魁蚶 Cu2+胁迫 生理生化 组织结构 |
| 收稿时间： | 2017/8/14 0:00:00 |
| 修稿时间： | 2018/1/31 0:00:00 |
Effect of Cu2+ stress on physiology biochemistry and histopathological structure of Scapharca broughtonii |
| |
| LI Yang,XUE Suyan,LI Jiaqi,SHEN Shufang,CHEN Qionglin,JIANG Zengjie,FANG Jianguang and MAO Yuze.Effect of Cu2+ stress on physiology biochemistry and histopathological structure of Scapharca broughtonii[J].Journal of Fisheries of China,2018,42(10):1531-1540. |
| |
| Authors: | LI Yang XUE Suyan LI Jiaqi SHEN Shufang CHEN Qionglin JIANG Zengjie FANG Jianguang and MAO Yuze |
| |
| Institution: | College of Fishers and Life Sciences, Shanghai Ocean University, Shanghai 201306, China;Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture, Shandong Provincial Key Laboratory of Fishery Resources and Eco-Environment, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China,Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture, Shandong Provincial Key Laboratory of Fishery Resources and Eco-Environment, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China;Laboratory for Marine Ecology and Environmental Science, Qingdao National Laboratory for Marine Science, Qingdao 266071, China,Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture, Shandong Provincial Key Laboratory of Fishery Resources and Eco-Environment, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China;Laboratory for Marine Ecology and Environmental Science, Qingdao National Laboratory for Marine Science, Qingdao 266071, China,College of Fishers and Life Sciences, Shanghai Ocean University, Shanghai 201306, China;Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture, Shandong Provincial Key Laboratory of Fishery Resources and Eco-Environment, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China,College of Fishers and Life Sciences, Shanghai Ocean University, Shanghai 201306, China;Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture, Shandong Provincial Key Laboratory of Fishery Resources and Eco-Environment, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China,Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture, Shandong Provincial Key Laboratory of Fishery Resources and Eco-Environment, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China,Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture, Shandong Provincial Key Laboratory of Fishery Resources and Eco-Environment, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China and Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture, Shandong Provincial Key Laboratory of Fishery Resources and Eco-Environment, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China;Laboratory for Marine Ecology and Environmental Science, Qingdao National Laboratory for Marine Science, Qingdao 266071, China |
| |
| Abstract: | |
| |
| Keywords: | Scapharca broughtonii Cu2+ stress physiological metabolism histopathological structure |
| 本文献已被 CNKI 等数据库收录！ |
| 点击此处可从《水产学报》浏览原始摘要信息 |
| 点击此处可从《水产学报》下载免费的PDF全文 |
|
