| 三疣梭子蟹核型分析 |
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| 引用本文： | 朱冬发.三疣梭子蟹核型分析[J].水产学报,2005,29(5):649-653. |
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| 作者姓名： | 朱冬发 |
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| 作者单位： | 宁波大学生命科学与生物工程学院,浙江,宁波,315211 |
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| 基金项目： | 浙江省自然科学基金资助项目（301213）;宁波市科技计划项目（01N40107-2）;宁波市青年基金项目（2004A620014） |
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| 摘 要： | 实验用三疣梭子蟹于2003年3月－2004年6月购自浙江象山石浦港。以成熟卵、精巢、胚胎及溞状幼体等为材料进行三疣梭子蟹染色体数目和核型的研究。染色体制片采用组织切片法和气干法，用Olympus显微镜进行观测、摄影，依据Levan等的染色体分类标准进行核型分析。结果表明，精巢最适宜进行三疣梭子蟹染色体计数，卵内溞状幼体最适宜做核型分析。三疣梭子蟹染色体的数目是2n=106，n=53。核型分析显示，三疣梭子蟹有20对（第1～20号）中部着丝粒染色体（m）、3对（第21－23号）亚中部着丝粒染色体（sm）和30对（笫24～53号）端部着丝粒染包体（t）。因此，三疣梭子蟹核型为2n=106=40m＋6sm＋60t，染色体臂数NF=152；没有检查出异形性染色体的存在。
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| 关 键 词： | 三疣梭子蟹 染色体 核型 |
| 文章编号： | 1000-0615（2005）05-0649-05 |
| 收稿时间： | 2004-10-14 |
| 修稿时间： | 2004年10月14 |
Karyotype analysis on Portunus trituberculatus |
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| ZHU Dong-fa.Karyotype analysis on Portunus trituberculatus[J].Journal of Fisheries of China,2005,29(5):649-653. |
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| Authors: | ZHU Dong-fa |
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| Institution: | Faculty of life Science and Biotechnology, Ningbo University, Ningbo 315211, China |
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| Abstract: | Individuals of Portunus trituberculatus were collected from Shipu Port,Xiangshan,Zhejiang Province from March 2003 to June 2004.The chromosomal number and karyotype of the swimming crab were studied by the tissue slice techniques applied to the mature oocytes and the air-drying method applied to the testes,embryos and zoeae.The spread plates were examined and the chromosomes at the mitotic or meiotic metaphase were photographed under an Olympus microscope.The chromosomes pairing were processed according to the relative lengths and the centromeric indexes.The chromosome pairs was classified following the method of Levan et al(1964).The results indicate that the testes are the best materials to be used in chromosome count and the egg-zoeae are the best materials to be used in karyotype analysis.The chromosomal numbers for P.trituberculatus are 2n=106,n=53.All chromosomes(2n=106) were matched in 53 pairs.They were divided into 3 groups.Group I contains 20 pairs(1st-20th) that are metacentric chromosomes(m).Group II has only 3 pairs(21st-23rd) of submetacentric chromosomes(sm).And group III includes 30 pairs(24th-53rd) which are telocentric chromosomes(t).Therefore,the karyotype formula of P.trituberculatus is 2n=106=40m 6sm 60t,NF=152.No sex-chromosome of heteromorphism was observed. |
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| Keywords: | Portunus trituberculatusPortunus trituberculatusPortunus trituberculatus chromosome karyotype |
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