| 大头鳕TNFSF6基因的结构分析及其在发育早期和病毒暴发时的表达水平 |
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| 引用本文： | 刘瑞婷,蒋洁兰,毛明光,姜志强,布尔特,矫志伟,陈明康,孙谦.大头鳕TNFSF6基因的结构分析及其在发育早期和病毒暴发时的表达水平[J].水产学报,2019,43(2):523-531. |
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| 作者姓名： | 刘瑞婷 蒋洁兰 毛明光 姜志强 布尔特 矫志伟 陈明康 孙谦 |
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| 作者单位： | 大连海洋大学, 农业农村部北方海水增养殖重点实验室, 辽宁省北方鱼类应用生物学与增养殖重点实验室, 辽宁 大连 116023,大连海洋大学, 农业农村部北方海水增养殖重点实验室, 辽宁省北方鱼类应用生物学与增养殖重点实验室, 辽宁 大连 116023,大连海洋大学, 农业农村部北方海水增养殖重点实验室, 辽宁省北方鱼类应用生物学与增养殖重点实验室, 辽宁 大连 116023;中国科学院水生生物研究所, 淡水生态与生物技术国家重点实验室, 湖北 武汉 430072,大连海洋大学, 农业农村部北方海水增养殖重点实验室, 辽宁省北方鱼类应用生物学与增养殖重点实验室, 辽宁 大连 116023,大连海洋大学, 农业农村部北方海水增养殖重点实验室, 辽宁省北方鱼类应用生物学与增养殖重点实验室, 辽宁 大连 116023,大连海洋大学, 农业农村部北方海水增养殖重点实验室, 辽宁省北方鱼类应用生物学与增养殖重点实验室, 辽宁 大连 116023,大连海洋大学, 农业农村部北方海水增养殖重点实验室, 辽宁省北方鱼类应用生物学与增养殖重点实验室, 辽宁 大连 116023,大连海洋大学, 农业农村部北方海水增养殖重点实验室, 辽宁省北方鱼类应用生物学与增养殖重点实验室, 辽宁 大连 116023 |
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| 基金项目： | 国家自然科学基金（31302202）；中国博士后基金（2016M602391）；中国水产科学研究院基本科研和农业部海洋渔业可持续发展重点实验室开放课题（2017HY-XKQ01-08）；辽宁省教育厅专项（QL201702，LR2016009） |
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| 摘 要： | 为揭示大头鳕TNFSF6基因的基本结构与功能及其在大头鳕发育和神经坏死病毒(Pacific cod nervous necrosis virus, PCNNV)暴发时的响应机制,本研究通过基因克隆获得TNFSF6 cDNA开放阅读框序列,并对序列进行生物信息学分析,运用相对荧光定量PCR(qRT-PCR)方法对TNFSF6在不同组织、孵化后不同日龄仔鱼和PCNNV感染前后仔稚鱼中的表达水平进行检测。结果显示,大头鳕TNFSF6 cDNA长1 388 bp,5′UTR占315bp,3′UTR占500 bp,ORF全长573 bp,编码190个氨基酸。qRT-PCR结果显示,TNFSF6在各组织均有表达,但在脾脏和鳃组织中的表达量较高;大头鳕孵化后15、20、37和40 d TNFSF6基因的转录水平分别是其在5 d转录水平的0.28、0.15、0.12和0.13倍。在24 d和46 d PCNNV暴发时,病鱼TNFSF6基因的转录水平高于对照组;在77 d PCNNV暴发时,病鱼TNFSF6转录水平则显著低于对照组。研究表明,TNFSF6基因在大头鳕发育早期和仔鱼暴发PCNNV时发挥了重要的作用。
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| 关 键 词： | 大头鳕 肿瘤坏死因子 TNFSF6 发育 神经坏死病毒 |
| 收稿时间： | 2017/10/10 0:00:00 |
| 修稿时间： | 2018/2/6 0:00:00 |
Structural analysis of TNFSF6 gene and its expression profiles in early development stages and virus outbreak in Gadus macrocephalus |
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| LIU Ruiting,JIANG Jielan,MAO Mingguang,JIANG Zhiqiang,BU Erte,JIAO Zhiwei,CHEN Mingkang and SUN Qian.Structural analysis of TNFSF6 gene and its expression profiles in early development stages and virus outbreak in Gadus macrocephalus[J].Journal of Fisheries of China,2019,43(2):523-531. |
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| Authors: | LIU Ruiting JIANG Jielan MAO Mingguang JIANG Zhiqiang BU Erte JIAO Zhiwei CHEN Mingkang and SUN Qian |
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| Institution: | Key Laboratory of Mariculture and Stock Enhancement in North China''s Liaoning Province Sea, Ministry of Agriculture and Rural Affairs, Key Laboratory of Fish Applied Biology and Aquaculture in North China, Liaoning Province, Dalian Ocean University, Dalian 116023, China,Key Laboratory of Mariculture and Stock Enhancement in North China''s Liaoning Province Sea, Ministry of Agriculture and Rural Affairs, Key Laboratory of Fish Applied Biology and Aquaculture in North China, Liaoning Province, Dalian Ocean University, Dalian 116023, China,Key Laboratory of Mariculture and Stock Enhancement in North China''s Liaoning Province Sea, Ministry of Agriculture and Rural Affairs, Key Laboratory of Fish Applied Biology and Aquaculture in North China, Liaoning Province, Dalian Ocean University, Dalian 116023, China;State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China,Key Laboratory of Mariculture and Stock Enhancement in North China''s Liaoning Province Sea, Ministry of Agriculture and Rural Affairs, Key Laboratory of Fish Applied Biology and Aquaculture in North China, Liaoning Province, Dalian Ocean University, Dalian 116023, China,Key Laboratory of Mariculture and Stock Enhancement in North China''s Liaoning Province Sea, Ministry of Agriculture and Rural Affairs, Key Laboratory of Fish Applied Biology and Aquaculture in North China, Liaoning Province, Dalian Ocean University, Dalian 116023, China,Key Laboratory of Mariculture and Stock Enhancement in North China''s Liaoning Province Sea, Ministry of Agriculture and Rural Affairs, Key Laboratory of Fish Applied Biology and Aquaculture in North China, Liaoning Province, Dalian Ocean University, Dalian 116023, China,Key Laboratory of Mariculture and Stock Enhancement in North China''s Liaoning Province Sea, Ministry of Agriculture and Rural Affairs, Key Laboratory of Fish Applied Biology and Aquaculture in North China, Liaoning Province, Dalian Ocean University, Dalian 116023, China and Key Laboratory of Mariculture and Stock Enhancement in North China''s Liaoning Province Sea, Ministry of Agriculture and Rural Affairs, Key Laboratory of Fish Applied Biology and Aquaculture in North China, Liaoning Province, Dalian Ocean University, Dalian 116023, China |
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| Abstract: | |
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| Keywords: | Gadus macrocephalus tumor necrosis factor TNFSF6 development viral nervous necrosis |
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