| 二倍体泥鳅与大鳞副泥鳅及杂交F1精子结构与活力 |
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| 引用本文： | 李猛,虞炯莹,王卫民.二倍体泥鳅与大鳞副泥鳅及杂交F1精子结构与活力[J].水产学报,2022,46(1):41-50. |
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| 作者姓名： | 李猛 虞炯莹 王卫民 |
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| 作者单位： | 华中农业大学水产学院,农业动物遗传育种与繁育教育部重点实验室,农业农村部淡水生物繁育重点实验室,湖北武汉430070 |
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| 基金项目： | 国家自然科学基金31372180 |
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| 摘 要： | 二倍体泥鳅与大鳞副泥鳅杂交能够顺利获得杂交后代,其正反交核型都为（2n=49）,但杂交后代精巢表现出明显的发育迟缓现象,杂交泥鳅自交与回交实验表明,其雄性不可育。为了解二倍体泥鳅和大鳞副泥鳅杂交F1雄性不育的原因,实验通过计算机辅助精子活力分析系统（CASA）、光学显微镜、扫描电镜、透射电镜、流式细胞技术分别对二倍体泥鳅（DD）、大鳞副泥鳅（PP）、其正反杂交F1（DP）和（PD） 4种泥鳅精子的形态结构与活力进行了分析。结果显示,激活30 s时,DD和PP的精子运动率分别为76.50%±0.70%和75.17%±8.60%,极显著高于DP （3.65%±1.75%）和PD （2.68%±0.63%）,且杂交泥鳅的精子的平均运动速度（VAP）、平均曲线速度（VCL）、平均直线运动速度（VSL）等运动参数也极显著低于DD和PP,说明杂交泥鳅精子的活力极其低下。应用流式细胞技术对4种泥鳅精巢内细胞进行倍性鉴定,发现DD和PP精巢内大多为1N精子,而DP和PD精巢内除了正常单倍体精子外,还有大量的2N和4N以及少量的8N细胞。通过扫描电镜和透射电镜...
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| 关 键 词： | 二倍体泥鳅 大鳞副泥鳅 远缘杂交 计算机辅助精子活力分析系统 流式细胞仪 扫描电镜 透射电镜 |
| 收稿时间： | 2020/10/2 0:00:00 |
| 修稿时间： | 2021/3/24 0:00:00 |
Structure and vitality of sperm of diploid M. anguillicaudatus, P. dabryanus and their hybrid F1 |
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| LI Meng,YU Jiongying,WANG Weimin.Structure and vitality of sperm of diploid M. anguillicaudatus, P. dabryanus and their hybrid F1[J].Journal of Fisheries of China,2022,46(1):41-50. |
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| Authors: | LI Meng YU Jiongying WANG Weimin |
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| Institution: | College of Fishery,Key Laboratory of Agricultural Animal Genetics,Breeding and Reproduction of Ministry of Education,Key Laboratory of Freshwater Animal Breeding,Ministry of Agriculture,Huazhong Agricultural University,,College of Fishery,Key Laboratory of Agricultural Animal Genetics,Breeding and Reproduction of Ministry of Education,Key Laboratory of Freshwater Animal Breeding,Ministry of Agriculture,Huazhong Agricultural University |
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| Abstract: | The loach M.anguillicaudatus (DD) (Cobitidae; Cobitinae; Misgurnus), and large scale loach P.dabryanus (PP) (Cobitidae; Cobitinae; Paramisgurnus), hybrid F1 generation (DP) and (PD) four loach sperm morphology and vitality are analyzed through computer assisted sperm analysis system (CASA), optical microscope, scanning electron microscopy (SEM), transmission electron microscopy (TEM) and flow cytometry technology in order to explore the reason of male sterility in diploid loach and large scale in vice loach hybrid F1 generation, preliminarily. The results showed that the sperm motility rates of DD and PP were 76.50 ± 0.70% and 75.17 ± 8.60% respectively at 30s of activation, which were significantly higher than DP (3.65 ± 1.75%) and PD (2.68 ± 0.63%) (P<0.01), and the related motion parameters of hybrid loach were also significantly lower than DD and PP (P < 0.01). Ploidy identification was carried out on the sperms of four species of lodges in the sperms of DD and PP by flow cytometers. It was found that the sperms of DD and PP were mostly 1N cell, while there were a large number of 2N and 4N and a small number of 8N cells in the sperms of DP and PD except for the normal haploid sperm. The spermatozoa in DD and PP spermatozoa were dense, intact and uniform in length (33.71 ± 2.12) and (34.28 ± 1.83) m, respectively, by SEM and TEM. DP and PD in the testis appeared a large number of abnormal sperm, such as multitailed sperm, binuclear sperm and big-head sperm. Head size showed obvious polymorphism, according to the head size measurements to estimate its ploidy, including normal sperm haploid sperm, at the same time have a diploid and tetraploid sperm, or even higher haploid sperm. The abnormal sperm of these hybrid loach provided important evidence for its low sperm motility and male sterility. |
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| Keywords: | Diploid Misgurnus anguillicaudatus Paramisgurnus dabryanus Distant hybridization CASA Flow cytometry Scanning electron microscope(SEM) Transmission electron microscopy(TEM) |
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