| 鲤gip克隆、序列分析及其基因表达的调节 |
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| 引用本文： | 杨国坤,徐双阳,梁晓敏,秦超彬,张艳敏,孟晓林,聂国兴.鲤gip克隆、序列分析及其基因表达的调节[J].水产学报,2022,46(1):1-9. |
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| 作者姓名： | 杨国坤 徐双阳 梁晓敏 秦超彬 张艳敏 孟晓林 聂国兴 |
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| 作者单位： | 河南师范大学水产学院,河南新乡453007;河南省水产动物养殖工程技术研究中心,河南新乡453007 |
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| 基金项目： | 国家自然科学基金（31872581, U1904118）；河南省重点科技攻关项目（202102110259）；河南省自然科学基金（212300410174） |
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| 摘 要： |
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| 关 键 词： | 鲤 Gip 鉴定 表达分析 |
| 收稿时间： | 2021/5/14 0:00:00 |
| 修稿时间： | 2021/8/6 0:00:00 |
Cloning and sequence analysis of common carp (Cyprinus carpio) gip and the regulation of its mRNA expression |
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| YANG Guokun,XU Shuangyang,LIANG Xiaomin,QIN Chaobin,ZHANG Yanmin,MENG Xiaolin,NIE Guoxing.Cloning and sequence analysis of common carp (Cyprinus carpio) gip and the regulation of its mRNA expression[J].Journal of Fisheries of China,2022,46(1):1-9. |
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| Authors: | YANG Guokun XU Shuangyang LIANG Xiaomin QIN Chaobin ZHANG Yanmin MENG Xiaolin NIE Guoxing |
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| Institution: | College of Fisheries, Henan Normal University,,,,,,College of Fisheries, Henan Normal University |
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| Abstract: | As the endocrine factor, incretin play a pivotal role in the regulation of glucose and lipid metabolism. As an important incretin, GIP are involved in glucose uptake and lipid deposition. To investigate the role of GIP in the common carp (Cyprinus carpio), a series of experiment was performed. The common carp gip were cloned from the foregut by RT-PCR, and the sequence was analyzed by bioinformatics and the mRNA expression was detected by Real-time PCR. The result showed that the ORF of common carp gip were 324 bp, which encode 107 amino acids. Based on amino acids sequence, the structure of GIP precursor protein contains four domains: signal peptide, N-terminal domain, mature GIP peptide and C-terminal domain. The results of sequence alignment and phylogenetic tree showed that the common carp GIP protein were closest to that of zebrafish. The Real-time PCR results indicated that the gip was widely expressed in multiple tissues, and was with higher expression in the intestine and pituitary of common carp. In OGTT experiment, the gip mRNA expression was significantly increased in common carp foregut by glucose treatment for 1 h and 2 h. In the fasting and re-feed experiment, the gip mRNA expression in the foregut was markedly decreased after fasting 7 days, and was significantly increased after re-feed. In the feeding experiment, the gip expression was increased in the high glucose and high lipid groups. The results will provide the data base for consummating GIP biological functions and theoretical foundation for investigating the regulatory of glucose and lipid metabolism in fish. |
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| Keywords: | common carp GIP identification expression analysis |
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