| 布氏石斑鱼的染色体核型、银染和C-带 |
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| 引用本文： | 蔡 岩,周永灿,谢瑞敏,谢珍玉,冯永勤,王世锋.布氏石斑鱼的染色体核型、银染和C-带[J].水产学报,2012,36(5):647-651. |
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| 作者姓名： | 蔡 岩 周永灿 谢瑞敏 谢珍玉 冯永勤 王世锋 |
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| 作者单位： | 1. 海南大学热带生物资源可持续利用省部共建国家重点实验室培育基地,海南海口570228;海南大学海洋生物实验教学示范中心,海南海口 570228
2. 海南大学热带生物资源可持续利用省部共建国家重点实验室培育基地,海南海口,570228 |
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| 基金项目： | 国家自然科学基金项目(31060355); 农业科技成果转化项目(2009GB2E200302); 海洋渔业科学与技术浙江省重中之重学科开放课题资助项目(20100115); 海南大学科研启动基金资助项目(kyqd1017); 海南大学“211工程”研究生科技创新平台建设项目 |
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| 摘 要： | 为了补充布氏石斑鱼细胞遗传学数据, 并为石斑鱼染色体进化研究以及石斑鱼遗传育种等工作奠定基础, 实验利用Giemsa染色、C-带和Ag-NORs方法对布氏石斑鱼的染色体核型以及异染色质和具有转录活性的核仁组织区在染色体上的分布位置和数量进行了研究。结果表明,布氏石斑鱼二倍体染色体数目为48, 其染色体组由48条端部着丝粒染色体组成, 绝大多数染色体的着丝粒位置呈异染色质带, 仅在第24对染色体靠近着丝粒的位置检测到银染带, 以上结果均表明, 布氏石斑鱼属于较为原始的类群。
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| 关 键 词： | 布氏石斑鱼 染色体 核型 银染 C-带 |
| 收稿时间： | 2011/9/16 0:00:00 |
| 修稿时间： | 2011/12/19 0:00:00 |
A study on the karyotype, Ag-NORs and C-banding in Epinephelus bleekeri |
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| CAI Yan,ZHOU Yong-can,XIE Rui-min,XIE Zhen-yu,FENG Yong-qin and WANG Shi-feng.A study on the karyotype, Ag-NORs and C-banding in Epinephelus bleekeri[J].Journal of Fisheries of China,2012,36(5):647-651. |
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| Authors: | CAI Yan ZHOU Yong-can XIE Rui-min XIE Zhen-yu FENG Yong-qin and WANG Shi-feng |
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| Institution: | 1.Hainan University, State Key Laboratory Breeding Base for Sustainable Exploitation of Tropical Biotic Resoures 2.Experimental Teaching Demonstration Center of Marine Biology,1.Hainan University, State Key Laboratory Breeding Base for Sustainable Exploitation of Tropical Biotic Resoures 2.Experimental Teaching Demonstration Center of Marine Biology,Hainan University, State Key Laboratory Breeding Base for Sustainable Exploitation of Tropical Biotic Resoures,Hainan University, State Key Laboratory Breeding Base for Sustainable Exploitation of Tropical Biotic Resoures,Hainan University, State Key Laboratory Breeding Base for Sustainable Exploitation of Tropical Biotic Resoures,Hainan University, State Key Laboratory Breeding Base for Sustainable Exploitation of Tropical Biotic Resoures |
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| Abstract: | Epinephelus bleekeri is important in commercial fisheries of tropical and subtropical seas. It has been broadly cultured in many Asian countries, such as China, Japan, Vietnam and Malaysia.Despite many studies on its culture techniques, the detailed cytogenetic data of this species is still lacking. To add E. bleekeri cytogenetic data and lay the foundation for grouper chromosome evolution and breeding studies, the karyotype of E. bleekeri was studied in this paper using multiple methods, including Giemsa staining, Ag-NORs and C-banding. The following results were obtained: E. bleekeri had a diploid chromosome number of 48 and its karyotype formula was 48t, NF=48, and sex chromosome was not observed; the constitutive heterochromatin was observed at the centromeric region on most chromosomes; but only a single pair of NORs was detected at the paracentromeric region of chromosome pair No.24. Based upon above results, it is concluded that E. bleekeri was relatively primitive among genus Epinephelus. |
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| Keywords: | Epinephelus bleekeri karyotype Ag-NORs C-bands |
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