| 菲律宾蛤仔稚贝最适生长环境条件的响应面法分析 |
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| 引用本文： | 桑士田,闫喜武,杨鹏,杨霏,梁健,刘振,霍忠明,张国范.菲律宾蛤仔稚贝最适生长环境条件的响应面法分析[J].水产学报,2012,36(9):1410-1417. |
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| 作者姓名： | 桑士田 闫喜武 杨鹏 杨霏 梁健 刘振 霍忠明 张国范 |
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| 作者单位： | 大连海洋大学水产与生命学院,辽宁省贝类良种繁育工程技术研究中心;大连海洋大学水产与生命学院,辽宁省贝类良种繁育工程技术研究中心;大连海洋大学水产与生命学院,辽宁省贝类良种繁育工程技术研究中心;大连海洋大学水产与生命学院,辽宁省贝类良种繁育工程技术研究中心;大连海洋大学水产与生命学院,辽宁省贝类良种繁育工程技术研究中心;大连海洋大学水产与生命学院,辽宁省贝类良种繁育工程技术研究中心;大连海洋大学水产与生命学院,辽宁省贝类良种繁育工程技术研究中心;中国科学院海洋研究所 |
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| 基金项目： | 现代农业体系建设专项(CARS-48) |
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| 摘 要： | 为考察温度(T)、盐度(S)、pH及三者交互作用对菲律宾蛤仔稚贝期壳长生长的影响,使用Design-Expert 7.0软件采用BBD(Box-Behnken Design)实验设计方案,进行响应面分析,寻找最适生长条件组合,并构造蛤仔稚贝壳长生长模型。实验进行30 d对蛤仔稚贝壳长(L)进行测量。实验条件梯度设计为温度(30℃、25℃、20℃)、盐度(15、20、25)、pH(7.00±0.09)、(8.00±0.09)、(9.00±0.09)]。结果显示:(1)温度在实验区间内与蛤仔稚贝壳长生长呈一定的正相关,在30℃附近达到壳长极值,盐度在实验区间内也与壳长生长呈一定正相关,但在接近25时达到壳长极值,pH在8～9时出现壳长极值,略偏碱性水体有利于蛤仔稚贝生长。(2)温度、盐度及pH三者间对蛤仔稚贝生长无显著交互作用(P>0.05)。通过Design-Expert 7.0软件对数据进行二次多元回归拟合,得到蛤仔稚贝壳长Y对编码自变量A、B和C的二次多元回归方程:Y=4.08+0.10A+0.18B+0.11C-0.055A2-0.13B2-0.12C2(R2=0.977 4),软件模拟最适蛤仔稚贝生长的条件组合为T=29.65℃,S=23.35,pH=(8.46±0.09)]。
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| 关 键 词： | 菲律宾蛤仔 温度 盐度 pH 响应面法分析 |
| 收稿时间： | 2011/11/15 0:00:00 |
| 修稿时间： | 2012/5/30 0:00:00 |
Response surface methodology for optimization of growth condition in juvenile Ruditapes philippinarum |
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| SANG Shi-tian,YAN Xi-wu,YANG Peng,YANG Fei,LIANG Jian,LIU Zhen,HUO Zhong-ming and ZHANG Guo-fan.Response surface methodology for optimization of growth condition in juvenile Ruditapes philippinarum[J].Journal of Fisheries of China,2012,36(9):1410-1417. |
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| Authors: | SANG Shi-tian YAN Xi-wu YANG Peng YANG Fei LIANG Jian LIU Zhen HUO Zhong-ming and ZHANG Guo-fan |
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| Institution: | 1.Engineering and Technology Research Centre of Shellfish Breeding in Liaoning Province, College of Fisheries and Life Science,Dalian Ocean University,Dalian 116023,China; 2.Institute of Oceanology,Chinese Academy of Sciences,Qingdao 266071,China) |
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| Abstract: | In this paper,the optimum temperature,salinity and pH for juvenile growth of Ruditapes philippinarum were determined through the response surface methodology(RSM).The multivariate non-linear model was applied to study the interactions and optimization of the growth parameters for R.philippinarum which were explored by experiments.The results showed that:(1)there were significantly positive correlations between growth of shell length and temperature and salinity within the range of experiment design.The relationship between growth of shell length and temperature and salinity and the growth of shell-length verged to positive correlation,and the calm grew better between pH 8 and 9;(2)there were no significant interactions between temperature,salinity and pH for the growth of juveniles.The multivariate non-linear model is as follows:Y=4.08+0.10A+0.18B+0.11C-0.055A2-0.13B2-0.12C2(R2=0.977 4),the optimum conditions for germination are as follows:temperature 29.65 ℃ and salinity 23.35‰ and pH=8.46±0.09.The experimental results indicated that the proposed model is reasonably accurate and can be used for describing and predicting the growth features of R.philippinarum in different environmental factors. |
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| Keywords: | Ruditapes philippinarum temperature salinity pH response surface methodology |
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