| 脊尾白虾3个野生群体ITS1序列分析及其亲缘关系分析 |
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| 引用本文： | 马 朋,刘 萍,李 健.脊尾白虾3个野生群体ITS1序列分析及其亲缘关系分析[J].水产学报,2012,36(8):1185-1192. |
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| 作者姓名： | 马 朋 刘 萍 李 健 |
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| 作者单位： | 1. 中国水产科学研究院黄海水产研究所,山东青岛266071;大连海洋大学水产与生命学院,辽宁大连 116023
2. 中国水产科学研究院黄海水产研究所,山东青岛,266071 |
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| 基金项目： | 国家“八六三“高技术研究发展计划项目资助(， 2012AA10A409)号; 国家虾产业技术体系(CARS-47)和; 山东省科技发展计划项目(2011GHY11526) |
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| 摘 要： | 对脊尾白虾的莱州湾、海洲湾、象山湾3个野生群体共计62个个体的核糖体RNA转录单元内间隔区ITS1基因片段进行克隆和测序,对序列特点进行分析,并结合GenBank数据库中已有的长臂虾亚科ITS1同源序列虾类进行系统分析。结果显示,脊尾白虾的ITS1序列具有长度多态性,其长度为345~384 bp,62条序列GC的平均含量显著高于AT含量;共检测到79个变异位点,39种单倍型,多态位点比例为21.7%;海洲湾群体遗传多样性指数最高,象山湾群体次之,莱州湾群体最低。在脊尾白虾ITS1序列中发现微卫星序列共有8处,重复序列类型为(GC)n、(AG)n、(GGC)n、(GGA)n、(AT)n、(GA)n,以(GA)n类型最多。AMOVA分析结果显示3个群体间的遗传分化较弱或只有中度分化。另外用MEGA4.0软件中的NJ法构建分子进化树,探讨长臂虾亚科几个种的系统进化关系,系统树显示同种的不同个体、同属的不同种聚在一起,与形态学的分类吻合。
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| 关 键 词： | 脊尾白虾 ITS1 遗传多样性 系统进化 |
| 收稿时间： | 1/4/2012 12:00:00 AM |
| 修稿时间： | 2012/2/26 0:00:00 |
The genetic diversity and phylogenetic analysis of ITS1 in mitochondrial DNA of three populations of Exopalaemon carinicauda |
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| MA Peng,LIU Ping and LI Jian.The genetic diversity and phylogenetic analysis of ITS1 in mitochondrial DNA of three populations of Exopalaemon carinicauda[J].Journal of Fisheries of China,2012,36(8):1185-1192. |
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| Authors: | MA Peng LIU Ping and LI Jian |
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| Institution: | 1(1.Yellow Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Qingdao 266071,China;2.College of Fisheries and Life Sciences,Ocean University of Dalian,Dalian 116023,China) |
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| Abstract: | The ribosomal DNA internal transcribed spacer 1 (ITS1) of three wild populations of Exopalaemon carinicauda was cloned and sequenced. The phylogenetic relationship among Palaemoninae was discussed using ITS1 from GenBank database. The results show that the length of the ITS1 ranged from 345 to 384 bp. The mean contents of GC were higher than AT;79 variation sites and 39 haplotypes were obtained in ITS1; Nucleotide diversity and average number of nucleotide diferences were calculated to analyze the gene sequence variation. Eight kinds of microsatellites were found in E. carinicauda. The type of (GA) n was rich in ITS1. AMOVA analysis results show that the genetic differentiation of the three groups was weak or only moderately differentiated.The molecular phylogenetic tree was constructed with NJ method using software MEGA 4.0 to study several species of the Palaemoninae molecular phylogeny. According to the NJ tree, different individuals of the same species, different species of the same genera were grouped together, in consistence with the morphological taxonomy. |
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| Keywords: | Exopalaemon carinicauda ITS1 genetics diversity Pphylogenetic |
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