| 克氏原螯虾血细胞及免疫功能的初步研究 |
| |
| 引用本文： | 陆剑锋,万全,吴旭干,杨筱珍,赵维信,成永旭.克氏原螯虾血细胞及免疫功能的初步研究[J].水产学报,2009,33(6):1018-1025. |
| |
| 作者姓名： | 陆剑锋 万全 吴旭干 杨筱珍 赵维信 成永旭 |
| |
| 作者单位： | 合肥工业大学生物与食品工程学院,上海海洋大学生命科学与技术学院,上海海洋大学生命科学与技术学院,上海海洋大学生命科学与技术学院 |
| |
| 基金项目： | 国家自然科学基金（30471349）; 上海市科委科技攻关计划(08dz1206002) |
| |
| 摘 要： | 甲壳动物血细胞在宿主免疫应答中发挥着重要的作用，其中包括识别、吞噬、包囊黑色素形成、胞毒作用和胞间的信息传递。不同类型的血细胞其防御机制不同，因此血细胞的形态和分类是甲壳动物免疫学的基础。通常情况，十足目甲壳动物的血细胞类型主要依据细胞质中颗粒物质的有无和颗粒物质的相对大小进行分类。研究采用Giemsa染色法、H.E染色法和电子显微技术法对克氏原螯虾血细胞进行分类，观察到3类血细胞：透明细胞、小颗粒细胞、大颗粒细胞，它们所占的比例分别为19.9%，46.1%，34.1%。在电镜下，这3类血细胞的超微结构极为容易辨识。透明细胞通常在3类细胞中最小，核质（N/C）比最高，但无明显的颗粒；相比之下，小颗粒细胞和大颗粒细胞内部的颗粒数量逐渐增多。利用体内和体外培养技术研究克氏原螯虾血细胞对外源体（以Sephadex A-25色谱硅珠作为抗原）的包囊作用，初步探讨克氏原螯虾血细胞在对外源体免疫应答过程中的识别机理。将Sephadex A-25色谱硅珠植入克氏原螯虾体内，抗原物周围被大量血细胞包囊，通过切片技术观察到抗原物周围的血细胞绝大部分为小颗粒细胞，证实在包囊过程中起主要作用的血细胞是小颗粒细胞，即小颗粒细胞在包囊反应中比较活跃。研究结果为以后进一步研究克氏原螯虾的细胞免疫机制和提高其抗病能力提供了科学依据。
|
| 关 键 词： | 克氏原螯虾 血细胞 免疫 包囊 |
| 收稿时间： | 6/8/2008 12:00:00 AM |
| 修稿时间： | 2008/10/11 0:00:00 |
A preliminary study on haemocytes and immune functions of the crayfish, Procambarus clarkii |
| |
| LU Jian-feng,Wan Quan,WU Xugan,YANG Xiao-zhen,ZHAO Weinxin and CHENG Yongxu.A preliminary study on haemocytes and immune functions of the crayfish, Procambarus clarkii[J].Journal of Fisheries of China,2009,33(6):1018-1025. |
| |
| Authors: | LU Jian-feng Wan Quan WU Xugan YANG Xiao-zhen ZHAO Weinxin and CHENG Yongxu |
| |
| Institution: | School of Biotechnology and Food Engineering, Hefei University of Technology,College of Aqua-life Science and Technology, Shanghai Ocean University,College of Aqua-life Science and Technology, Shanghai Ocean University and College of Aqua-life Science and Technology, Shanghai Ocean University |
| |
| Abstract: | Crustacean haemocytes play important roles in the host immune response including recognition, phagocytosis, encapsulation melanization, cytotoxicity and cell-cell communication. The various types of haemocytes are responsible for a number of defense mechanisms. Thus, the morphology and classification of haemocytes are the basis of crustacean immunology. Generally, classification of the haemocyte types in decapod crustaceans is based mainly on the presence of cytoplasmic granules and relative size of granules. In this paper, Giemsa s staining, hematoxylin and eosin (H-E) staining and electron microscope techniques were used to classify the haemocytes of Procambarus clarkii. And three types of haemocytes, including hyaline cell (HC), semi-granular cell(SGC) and granular cell(GC), were observed, accounting for 19.9% ,34.1% ,46.1% respectively. In addition, the ultrastructures of haemocytes turned out to be most easily recognized under the electron microscope. The hyaline cells were generally the smallest cells with the highest nuclear-cytoplasmatic (N/C) ratio, containing few granules, while the semi-granulocytes and the granulocytes had increasing amount of granules inside their cells. Afterwards using in vivo and in vitro incubation methods, the haemocyte immunology responses of P. Clarkii were investigated, with Sephadex A-25 chromatography beads as the antigens. Meanwhile, the cellular recognition mechanism during the immune responses was also preliminarily discussed. When the Sephadex A-25 beads were transplanted into the body of P. Clarkii for a certain period of time, a large number of haemocytes, which were almost recognized as semi-granular cells with slice technique, encapsulated the antigens. It was proved that semi-granular cells played a prominent role during the encapsulation process. Therefore, the semi-granular cells were the cells active in encapsulation responses. Besides, the results also laid a scientific foundation for the further study of cellular immunological mechanism and anti-disease ability of P. Clarkii. |
| |
| Keywords: | Procambarus clarkii haemocytes immunity encapsulation |
| 本文献已被 万方数据 等数据库收录！ |
| 点击此处可从《水产学报》浏览原始摘要信息 |
| 点击此处可从《水产学报》下载免费的PDF全文 |
|
