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| 黄姑鱼群体遗传多样性的AFLP分析 | |
| 引用本文： | 韩志强.黄姑鱼群体遗传多样性的AFLP分析[J].水产学报,2006,30(5):640-646. |
| 作者姓名： | 韩志强 |
| 作者单位： | 1. 中国海洋大学教育部海水养殖重点实验室,山东,青岛,266003;中国水产科学研究院黄海水产研究所,山东,青岛,266071 2. 中国海洋大学教育部海水养殖重点实验室,山东,青岛,266003 3. 集美大学水产学院,福建,厦门,361021 4. 中国水产科学研究院黄海水产研究所,山东,青岛,266071 5. 中国水产科学研究院水产种质资源与养殖技术重点开放实验室,广东,广州,510300 |
| 基金项目： | 国家自然科学基金;国家重点基础研究发展计划(973计划);中国水产科学研究院水产种质资源与养殖技术重点开放实验室基金 |
| 摘 要： | 对青岛和厦门黄姑鱼群体的遗传多样性进行了AFLP分析，5对选择性引物在两个群体47个个体中，共扩增出461个位点，多态位点265个。青岛和厦门群体的多态位点比例、Nei遗传多样性指数和Shannon遗传多样性指数分别为51.70%、51.99%，0.1022、0.0996，0.1643、0.1622；两个群体遗传多样性在同一水平上。基因分化系数Gst、Shannon遗传多样性指数和AMOVA分析均显示黄姑鱼的遗传变异主要来源于群体内个体间，而群体间无明显的遗传分化。群体的显性基因型频率分布和位点差异数分布显示两个群体有基本相同的群体遗传结构。结果表明，黄姑鱼青岛和厦门群体间无明显的遗传差异，群体间有明显的基因交流。 |
| 关 键 词： | 黄姑鱼 遗传多样性 |
| 文章编号： | 1000-0615（2006）05-0640-07 |
| 收稿时间： | 2006-01-25 |
| 修稿时间： | 2006-01-25 |
Analysis of genetic diversity of Nibea albiflora by AFLP markers |
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| HAN Zhiqiang.Analysis of genetic diversity of Nibea albiflora by AFLP markers[J].Journal of Fisheries of China,2006,30(5):640-646. | |
| Authors: | HAN Zhiqiang |
| Institution: | Ocean University of China,Qingdao |
| Abstract: | Nibea albiflora is a commercially important marine fish species,which is widely distributed in Chinese,Japanese and Korean waters.Nibea albiflora has been intensively studied in China,including its basic biological characteristics and artificial reproduction.However,very few studies on N.albiflora were focused on genetic diversity,which has become an obstacle to further researches on the protection,management,rational exploitation and sustainable utilization of N.albiflora resources.The yield of N.albiflora has declined quickly due to increased efficiency of capture techniques and the leap of catch intensity.The information of genetic diversities of different populations will give us theoretical guidance in breeding and genetic improvement.In this study,a new molecular techonology,amplified fragment length polymorphism(AFLP) was used to analyze the genetic diversity and variation of two populations of N.albiflora from Qingdao and Xiamen sea areas.Procedures of AFLP analysis were essentially based on Vose et al.and Wang et al. A total of 47 individuals were studied using five primer combinations(E-AAC/MCAG,E-AGG/M-CTT,E-AGG/M-CAG,E-AGC/M-CAT,E-AGC/M-CAG).AFLP bands were scored for presence(1) or absence(0) and transformed into 0/1 binary character matrix.AMOVA and mismatch distribution analysis were performed in Arlequin,Nei genetic diversity and Shannon genetic diversity index were calculated in POPGEN.A total of 461 loci ranging in size from 60 bp to 550 bp were detected from 47 individuals,of which 265 were polymorphic.The number of bands per primer combination varied from 66 to 126 and the percentage of polymorphic bands per primer combination ranged from 52.58% to 62.70%.The proportion of polymorphic loci,the Nei genetic diversity and Shannon genetic diversity index of Qingdao and Xiamen populations were 51.70% and 51.99%,0.1022 and 0.0996,0.1643 and 0.1622,respectively.The results showed that the genetic diversity of these two populations was at the same level.G_(st) value,Shannon genetic diversity index and AMOVA analysis indicated that the genetic variation mainly came from individuals within populations and there was no significant genetic differentiation between populations.The UPGMA tree based on AFLP data supported this result and no distinct clade was detectd.Mismatch distribution analysis and dominant gene frequency revealed that these two populations have the same genetic population structure.The results of AFLP analysis indicated that there is no significant genetic differentiation between two populations due to frequent gene flow.At present,the natural populations of marine fish,including N.albiflora,have been destroyed due to over exploitation and increasing marine pollution which caused the number of parent fish to be extremely diminished.These results provide information on the genetic structure of N.albiflora,which can be utilized to make informed management decisions.However,AFLP markers are inherited as dominant markers,further studies utilizing co-dominant markers are needed for a better understanding of the genetic diversity of this fish. |
| Keywords: | AFLP |
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