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| 基于16S rRNA部分序列探讨12种鲹科鱼类的分子系统进化关系 | |
| 引用本文： | 郑文娟,朱世华,邹记兴,杨迎春,沈锡权.基于16S rRNA部分序列探讨12种鲹科鱼类的分子系统进化关系[J].水产学报,2008,32(6):847-854. |
| 作者姓名： | 郑文娟 朱世华 邹记兴 杨迎春 沈锡权 |
| 作者单位： | 1. 宁波大学应用海洋生物技术教育部重点实验室,浙江,宁波,315211;宁波大学生命科学与生物工程学院生物系,浙江,宁波,315211 2. 华南农业大学动物科学学院,广东,广州,510642 3. 宁波大学应用海洋生物技术教育部重点实验室,浙江,宁波,315211 |
| 摘 要： | 鲹科鱼类在传统形态分类与分子遗传水平构建的系统分类存在争议，本文通过PCR扩增获得了鲹科（Carangidae）8属9种的线粒体16S rRNA基因片段序列约598bp碱基，结合来自GenBank的3种鲹科鱼类的相应片段序列，并以大斑石鲈Pomadasys maculates为外群，生成供系统发育分析的序列矩阵，利用MEGA version 3.0软件分析序列的碱基组成、差异百分比和转换/颠换值等，应用最大简约法和邻接法构建系统树。结果显示：（1）支持鲹科下设四个亚科（鲹亚科，鰤亚科，鲳鲹亚科，鰆鲹亚科）阶元的分类系统；（2）所测种类鲹亚科鲹属下不宜设亚属分类阶元；（3）及达副叶鲹与丽叶鲹亲缘关系近，16S rRNA基因片段序列碱基只有1.07%的差异，未达到分属水平，应同属于副叶鲹属的两个不同种，并建议丽叶鲹的中文名用“丽副叶鲹”。 |
| 关 键 词： | 鲹科 线粒体DNA 16S rRNA序列 系统发育 |
| 收稿时间： | 2007/9/22 0:00:00 |
| 修稿时间： | 2008/1/23 0:00:00 |
Molecular phylogenetic relationship of Carangidae based on partial sequence of mitochondrial 16S ribosomal RNA gene |
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| ZHENG Wen-Juan,ZHU Shi-Hu,ZOU Ji-Xing,YANG Ying-Chun and SHEN Xi-Quan.Molecular phylogenetic relationship of Carangidae based on partial sequence of mitochondrial 16S ribosomal RNA gene[J].Journal of Fisheries of China,2008,32(6):847-854. | |
| Authors: | ZHENG Wen-Juan ZHU Shi-Hu ZOU Ji-Xing YANG Ying-Chun and SHEN Xi-Quan |
| Institution: | Key Laboratory of Applied Marine Biotechnology (Ningbo University), Ministry of Education,Key Laboratory of Applied Marine Biotechnology (Ningbo University), Ministry of Education,,Key Laboratory of Applied Marine Biotechnology (Ningbo University), Ministry of Education,Key Laboratory of Applied Marine Biotechnology (Ningbo University), Ministry of Education |
| Abstract: | The classification and evolutionary relationship of Carangidae have remained much controversy between conventional morphological studies and molecular phylogenetic studies.In this study,the 16S ribosomal RNA partial sequences of 9 species of 8 genera from Carangidae in China sea were amplified using PCR technique.Approximately,598 bp gene fragments were obtained.The resulting data were combined with Pomadasys maculates designed outgroup and additional homologous sequences of 3 species from Carangidae downlo... |
| Keywords: | Carangidae Mitochondrial DNA 16S ribosomal RNA molecular phylogeny |
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