| 虾夷扇贝EST-SSR标记在栉孔扇贝中的通用性研究 |
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| 作者姓名： | 张广明 孙秀俊 吴彪 杨爱国 刘志鸿 周丽青 刘寒苗 赵庆 |
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| 作者单位： | 农业农村部海洋渔业可持续发展重点实验室中国水产科学研究院黄海水产研究所;青岛海洋科学与技术试点国家实验室海洋渔业科学与食物产出过程功能实验室;上海海洋大学水产与生命学院 |
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| 摘 要： | 为研究虾夷扇贝(Patinopecten yessoensis)和栉孔扇贝(Chlamys farreri)的群体遗传多样性并分析其亲缘关系,对在虾夷扇贝中开发的60个EST-SSR位点在栉孔扇贝中的通用性进行了研究。结果显示,21个位点可在栉孔扇贝中扩增出特异性条带,通用性比例达35.00%,其中,17个位点具有多态性,多态性比例达28.33%。在虾夷扇贝和栉孔扇贝群体中,平均等位基因数(N_a)分别为2.7647和2.3529;平均有效等位基因数(N_e)分别为1.9487和1.6350;平均观测杂合度(H_o)为0.6314和0.3333;平均期望杂合度(H_e)为0.4569和0.3139;平均多态信息含量(PIC)为0.3726和0.2597;Nei's(1973)基因多样性指数(I)分别为0.4493和0.3087;Shannon信息指数分别为0.7176和0.5041;固定指数(F_(is))检测发现,7个位点偏离了Hardy-Weinberg平衡;遗传分化系数(F_(st))为0.2398。本研究开发的21对通用性EST-SSR标记,为进一步开展虾夷扇贝和栉孔扇贝遗传多样性分析、分子辅助育种、基因发掘和种质资源评价奠定了基础。
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| 关 键 词： | 虾夷扇贝 栉孔扇贝 EST-SSRs 微卫星 多态性 通用性 |
| 收稿时间： | 2017/5/3 0:00:00 |
| 修稿时间： | 2017/5/16 0:00:00 |
Transferability of EST-SSR from Patinopecten yessoensis into Chlamys farreri |
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| Authors: | ZHANG Guangming SUN Xiujun WU Biao YANG Aiguo LIU Zhihong ZHOU Liqing LIU Hanmiao and ZHAO Qing |
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| Institution: | Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture and Rural Affairs, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071; Laboratory for Marine Fisheries Science and Food Production Processes, Pilot National Laboratory for Marine Science and Technology (Qingdao),
Qingdao 266071; College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306,Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture and Rural Affairs, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071; Laboratory for Marine Fisheries Science and Food Production Processes, Pilot National Laboratory for Marine Science and Technology (Qingdao),
Qingdao 266071,Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture and Rural Affairs, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071; Laboratory for Marine Fisheries Science and Food Production Processes, Pilot National Laboratory for Marine Science and Technology (Qingdao),
Qingdao 266071,Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture and Rural Affairs, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071; Laboratory for Marine Fisheries Science and Food Production Processes, Pilot National Laboratory for Marine Science and Technology (Qingdao),
Qingdao 266071,Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture and Rural Affairs, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071; Laboratory for Marine Fisheries Science and Food Production Processes, Pilot National Laboratory for Marine Science and Technology (Qingdao),
Qingdao 266071,Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture and Rural Affairs, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071; Laboratory for Marine Fisheries Science and Food Production Processes, Pilot National Laboratory for Marine Science and Technology (Qingdao),
Qingdao 266071,Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture and Rural Affairs, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071; Laboratory for Marine Fisheries Science and Food Production Processes, Pilot National Laboratory for Marine Science and Technology (Qingdao),
Qingdao 266071; College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306 and Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture and Rural Affairs, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071; Laboratory for Marine Fisheries Science and Food Production Processes, Pilot National Laboratory for Marine Science and Technology (Qingdao),
Qingdao 266071; College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306 |
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| Abstract: | |
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| Keywords: | Patinopecten yessoensis Chlamys farreri EST-SSRs Microsatellites Polymorphism Transferability |
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