| 栉江珧(Atrina pectinata)EST-SSR标记的开发与应用 |
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| 作者姓名： | 李东明 杨爱国 吴 彪 孙秀俊 周丽青 刘寒苗 张广明 |
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| 作者单位： | 1. 上海海洋大学水产与生命学院 上海201306;农业部海洋渔业可持续发展重点实验室中国水产科学研究院黄海水产研究所 青岛 266071;2. 农业部海洋渔业可持续发展重点实验室中国水产科学研究院黄海水产研究所 青岛 266071 |
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| 基金项目： | 山东省重点研发计划项目(2016GSF115012)、青岛市战略性新兴产业培育计划项目(13-4-1-60-hy)和科技基础条件平台项目(2060503-01)共同资助 |
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| 摘 要： | 本研究以前期获得的栉江珧(Atrina pectinata)转录组数据为基础,筛选获得10550条微卫星标记(SSR),检出率为8.2％,平均9.01 kb出现1个SSR位点.设计并合成120对SSR引物,筛选得到36对能够稳定扩增的引物,并利用已获得的36对SSR引物对青岛海区栉江珧进行了群体遗传多样性分析.结果显示,12对引物的扩增片段表现出多态性,共产生42个等位基因,平均每个位点产生3.5个等位基因,平均观测杂合度(Ho)、平均期望杂合度(He)和平均多态信息含量(PIC)分别为0.417、0.604和0.526,表明青岛海区栉江珧群体的遗传多样性较高.本研究获得的EST-SSR标记和群体遗传信息为栉江珧的种质资源保护提供了重要的参考资料.
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| 关 键 词： | 栉江珧 EST-SSR 遗传多样性 杂合度 |
| 收稿时间： | 2015/11/16 0:00:00 |
| 修稿时间： | 2015/12/23 0:00:00 |
Development and Application of the EST-SSR Markers in Atrina pectinata |
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| Authors: | LI Dongming YANG Aiguo WU Biao SUN Xiujun ZHOU Liqin LIU Hanmiao and ZHANG Guangming |
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| Institution: | College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306;Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071,Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071,Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071,Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071,Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071,College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306;Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071 and College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306;Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071 |
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| Abstract: | |
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| Keywords: | Atrina pectinate EST-SSR Genetic diversity Heterozygosity |
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