| 磺胺二甲嘧啶对中国明对虾(Fenneropenaeus chinensis)非特异性免疫酶活性的影响 |
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| 作者姓名： | 孙 铭 葛倩倩 李 健 葛红星 翟倩倩 赵法箴 |
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| 作者单位： | 1. 中国海洋大学水产学院 青岛 266003; 农业部海洋渔业可持续发展重点实验室 中国水产科学研究院黄海水产研究所 青岛 266071;2. 农业部海洋渔业可持续发展重点实验室 中国水产科学研究院黄海水产研究所 青岛 266071; 青岛海洋科学与技术国家实验室 海洋渔业科学与食物产出过程功能实验室 青岛 266071; 中国海洋大学水产学院 青岛 266003;3. 农业部海洋渔业可持续发展重点实验室 中国水产科学研究院黄海水产研究所 青岛 266071 |
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| 基金项目： | 国家虾产业技术体系专项(CARS-47)、山东省自主创新专项(2013CXC80202)、国家高技术研究发展计划“主要养殖甲壳类良种培育”(2012AA10A409)、2013年中国水产科学研究院院级基本科研业务费专项“鲆蝶鱼类磺胺药代谢模式及种间评估”(2013A1102)和公益性行业(农业)科研专项(201103034)共同资助 |
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| 摘 要： | 以50、100和150 mg/kg 磺胺二甲嘧啶连续投喂中国明对虾(Fenneropenaeus chinensis)5 d，于给药期间第1、2、3、4、5天及停止投喂药物的第1、2、3、4、5、7、10天取样，测定中国明对虾免疫相关指标超氧化物歧化酶(SOD)、碱性磷酸酶(AKP)、溶菌酶(LZM)和酚氧化酶(PO)活性的变化情况。结果显示，磺胺二甲嘧啶不同给药剂量对酶活性的作用效果不同，投喂渔药期间(0–5 d)，低浓度组 SOD 和 PO 活性均极显著高于对照组(P<0.01)；AKP 活性于投药第2、3天极显著低于对照组(P<0.01)，随后逐渐升高，于第5天显著高于对照组(P<0.05)；LZM 活性于投药第3、4天显著低于对照组(P<0.05)，于第5天极显著低于对照组(P<0.01)。中浓度组 SOD 活性在前2 d 显著高于对照组(P<0.05)，投药3、4 d 显著低于对照组(P<0.05)；AKP 活性于第5天活性最高，为对照组的1.14倍；LZM 活性在3、4、5 d 显著低于对照组(P<0.05)；PO 活性在投喂药物前期1–3 d 呈上升趋势，极显著高于对照组(P<0.01)，于第3天达到最高值。高浓度组 SOD 和 LZM 活性在投药期间显著低于对照组(P<0.05)；AKP 活性于投药期间显著高于对照组(P<0.05)；PO 活性于1、2 d 极显著高于对照组(P<0.01)，随后逐渐下降。停止投喂药物阶段(6–15 d)，3个浓度组各免疫相关指标均恢复至对照组水平。研究表明，低浓度磺胺二甲嘧啶对中国明对虾的免疫机能具有一定的影响，高浓度磺胺二甲嘧啶对中国明对虾的免疫机能具有明显的抑制作用。在使用抗菌药物进行抑菌或杀菌的同时，要综合考虑所选择给药剂量对对虾生理机能的影响。
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| 关 键 词： | 磺胺二甲嘧啶 中国明对虾 非特异性免疫 |
| 收稿时间： | 2015/7/3 0:00:00 |
| 修稿时间： | 2015/8/20 0:00:00 |
Effects of Sulfamethazine on the Non-Specific Immune Enzyme Activities of Fenneropenaeus chinensis |
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| Authors: | SUN Ming GE Qianqian LI Jian GE Hongxing ZHAI Qianqian and ZHAO Fazhen |
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| Institution: | Fisheries College, Ocean University of China, Qingdao 266003; . Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071,Fisheries College, Ocean University of China, Qingdao 266003; . Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071,Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071; Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266071,Fisheries College, Ocean University of China, Qingdao 266003; . Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071,Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071 and Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071 |
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| Abstract: | Shrimp production has been a well-developed pillar of the aquaculture industry in China. Fenneropenaeus chinensis, one of highly valuable shrimp species, was mainly raised in the Bohai Sea and the Yellow Sea in North China. Antimicrobials are most frequently applied during intensive aquacultures to prevent or cope with disease outbreaks. However, antimicrobials may also have toxic effects on cultured animals such as immunosuppression. To better understand the mechanisms, we measured activities of superoxide dismutase (SOD), alkaline phosphatase (AKP), lysozyme (LZM), and phenoloxidase (PO) in serum upon the treatment with sulfamethazine (SM2) at different concentrations. It was found that SM2 significantly enhanced SOD activity at 50 mg/kg, whereas inhibited SOD at 150 mg/kg. At 100 mg/kg, SM2 first boosted SOD activity in the first 2 days and then suppressed it from Day 3 of treatment compared to the control group. Treated with 50 mg/kg SM2, the activity of AKP was inhibited from Day 2 of treatment till Day 2 after the treatment, followed by a recovery to the control level. When the SM2 concentration was 100 mg/kg, the AKP activity was elevated to a peak value on Day 5 of the treatment and then dropped back to the control level. In the treatment with 150 mg/kg SM2, AKP activity altered in the same pattern as the medium concentration group. SM2 had inhibitive effects on LZM at 50, 100, and 150 mg/kg. In spite of the concentrations of SM2, the PO activity showed the same trend that it rose to a higher level than the control followed by a decrease. The non-specific immunity was inhibited by SM2 at 150 mg/kg. Therefore, the dosage of antibiotics should be carefully evaluated to ensure healthy aquaculture of shrimp. |
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| Keywords: | Sulfamethazine Fenneropenaeus chinensis Non-specific immunity |
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