| 两种壳色虾夷扇贝的RAPD分析 |
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| 作者姓名： | 孙秀俊 杨爱国 刘志鸿 周丽青 |
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| 作者单位： | 1. 农业部海洋渔业资源可持续发展利用重点实验室,中国水产科学研究院黄海水产研究所,青岛,266071;上海海洋大学,生命科学与技术学院,201306
2. 农业部海洋渔业资源可持续发展利用重点实验室,中国水产科学研究院黄海水产研究所,青岛,266071 |
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| 基金项目： | 国家高技术研究发展计划项目，国家科技支撑计划专题，国家自然科学基金 |
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| 摘 要： | 采用RAPD技术对两种壳色虾夷扇贝Patinopecten yessoensis的遗传多样性和遗传结构及其分化进行研究。用筛选出的22个随机引物对白色贝和褐色贝各40个个体进行RAPD扩增，进行群体内及群体间的遗传学分析。白色贝共检测出128个多态位点，多态位点的比例为79．5％，Shannon遗传多样性指数为0．424；褐色贝共检测出127个多态位点，多态位点的比例为78．9％，Shannon遗传多样性指数为0．423。白色贝和褐色贝之间的遗传相似性指数和遗传距离为0．961和0．039，二者之间的遗传分化指数Gst为0．052，遗传分化的程度较低。结果表明，白色贝和褐色贝之间的等位基因频率、多态位点的比例和遗传多样性等的差别不明显，遗传变异主要来自于群体内。S285—1在褐色贝大部分个体中都能获得扩增片段，但在白色贝所有个体中均未见这个位点的扩增片段，推断S285—1为白色贝的特异阴性片段。
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| 关 键 词： | 虾夷扇贝 壳色 RAPD 遗传多样性 遗传距离 |
| 收稿时间： | 2008/9/3 0:00:00 |
| 修稿时间： | 2008/11/28 0:00:00 |
RAPD analysis of genetic diversity of Patinopecten yessoensis with different shell colours |
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| Authors: | SUN Xiu-jun YANG Ai-guo LIU Zhi-hong ZHOU Li-qing |
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| Institution: | SUN Xiu-jun, YANG Ai-guo, LIU Zhi-hong, ZHOU Li-qing(1.Key laboratory for sustainable utilization of Marine Fisheries Resources,Ministry of Agriculture, Yellow Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences, Qingdao 266071;2. Shanghai Ocean University, College of Aqua-life Science and Technology, 201306) |
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| Abstract: | The genetic diversity,heredity structure and differentiation of two Patinopecten yessoensis populations of different shell colours(white and brown)were compared using RAPD method(Random Amplified Polymorphic DNA).A total of 161 interpretable bands ranging from 100-3 000 bp in each population were obtained by 22 arbitrary primers.The numbers of polymorphic loci were 128 and 127 for white scallops and brown scallops,respectively with the percentages of polymorphism 79.5% and 78.9% and Shannon information indices 0.424and 0.423,respectively.The high similarity rate(0.961)and low genetic differentiation(Gst=0.052)indicated that there was no significant difference in genetic differentiation and genetic structure between the two populations.According to the Shannon information index,the genetic diversity was mainly due to individual differences in each population.The band at locus S285-1 appeared in 30 of 40 individuals of brown scallops,but it disappeared completely with no amplification in white scallops; it is suggested that this band is the negative genetic marker in white scallops. |
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| Keywords: | RAPD Patinopecten yessoensis Shell colors RAPD Genetic diversity Genetic distance |
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