| 银鲫crh基因的克隆、组织表达谱及其对摄食的影响 |
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| 作者姓名： | 周朝伟 雷骆 邓星星 袁登越 杨旻珉 曹高祥 朱海星 李岩 朱成科 李代金 唐仁军 张桂众 李路宽 郑宗林 |
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| 作者单位： | 西南大学动物科学学院水产系 重庆 402460;内江师范学院生命科学院 内江 641100;重庆市梁平区水产站 重庆 405200 |
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| 基金项目： | 中央高校基本科研业务费专项资金(XDJK2016C047)资助 |
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| 摘 要： | 为研究crh (Corticotropin-releasing hormone)基因对鱼类摄食的调控作用,首次克隆了银鲫(Carassius auratus gibelio) crh基因c DNA全长序列。运用实时荧光定量PCR(RT-qPCR)技术检测crh基因m RNA在不同组织的表达及餐前、餐后和禁食对其表达的影响。结果显示,银鲫crh基因的c DNA序列全长为920 bp,其中,5’-UTR为53 bp,3’-UTR为378 bp,开放阅读框(Open reading frame,ORF)为489 bp。推导银鲫crh基因的ORF区编码蛋白由162个氨基酸组成,其中,含有11个氨基酸的保守区,24个氨基酸的信号肽,41个氨基酸的成熟肽。氨基酸序列的多重比较分析显示,银鲫crh基因与金鱼(Carassiusauratus)的同源性为99%,与鲤(Cyprinuscarpio)的同源性为96%。荧光定量PCR表达分析显示,银鲫crh基因在下丘脑中的表达量最高,其次是端脑和心脏。crh基因的表达量在餐前与餐后没有显著变化(P0.05),在禁食第5天、第7天出现极显著降低(P0.01),而在复投喂后第9天、第11天出现极显著升高(P0.01)。以上结果显示,crh基因在银鲫摄食调控方面可能扮演着重要角色。
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| 关 键 词： | 银鲫 crh基因 RT-qPCR 基因表达 摄食调控 |
| 收稿时间： | 2018/3/12 0:00:00 |
| 修稿时间： | 2018/4/15 0:00:00 |
Cloning, tissue expression profiling, functional characterization of corticotropin-releasing hormone (crh) on feeding in gibel carp (Carassius auratus gibelio) |
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| Authors: | ZHOU Chaowei LEI Luo DENG Xingxing YUAN Dengyue YANG Minmin CAO Gaoxiang ZHU Haixing LI Yan ZHU Chengke LI Daijin TANG Renjun ZHANG Guizhong LI Lukuan and ZHENG Zonglin |
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| Institution: | Department of Aquaculture, College of Animal Science, Southwest University Rongchang Campus, Chongqing 402460,Department of Aquaculture, College of Animal Science, Southwest University Rongchang Campus, Chongqing 402460,Department of Aquaculture, College of Animal Science, Southwest University Rongchang Campus, Chongqing 402460,Department of Aquaculture, College of Life Sciences, Neijiang Normal University. Neijiang 641100,Department of Aquaculture, College of Animal Science, Southwest University Rongchang Campus, Chongqing 402460,Department of Aquaculture, College of Animal Science, Southwest University Rongchang Campus, Chongqing 402460,Department of Aquaculture, College of Animal Science, Southwest University Rongchang Campus, Chongqing 402460,Department of Aquaculture, College of Animal Science, Southwest University Rongchang Campus, Chongqing 402460,Department of Aquaculture, College of Animal Science, Southwest University Rongchang Campus, Chongqing 402460,Department of Aquaculture, College of Animal Science, Southwest University Rongchang Campus, Chongqing 402460,Fisheries Adminstration of Liangping, Chongqing 405200,Fisheries Adminstration of Liangping, Chongqing 405200,Department of Aquaculture, College of Animal Science, Southwest University Rongchang Campus, Chongqing 402460 and Department of Aquaculture, College of Animal Science, Southwest University Rongchang Campus, Chongqing 402460 |
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| Abstract: | |
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| Keywords: | Carassius auratus gibelio crh gene RT-qPCR Gene expression Feeding regulation |
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