| 魁蚶4个地理群体遗传多样性微卫星分析 |
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| 作者姓名： | 田吉腾 刘志鸿 杨爱国 吴彪 周丽青 |
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| 作者单位： | [1]农业部海洋渔业可持续发展重点实验室中国水产科学研究院黄海水产研究所,青岛266071 [2]上海海洋大学水产与生命学院,201306 |
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| 基金项目： | 国家科技基础条件平台项目“黄渤海区水生生物种质资源标准化整理、整合与共享”、山东省科技发展计划项目（2010GHY10513）和中央级公益性科研院所基本科研业务费项目（2010-ts-07）共同资助 |
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| 摘 要： | 利用多态性好的20对微卫星引物，对中国日照、黄岛、蓬莱和韩国的4个魁蚶地理群体进行了遗传多样性分析。结果显示，20个位点在4个群体中的等位基因数为3～17，平均等位基因数为8．35，平均有效等位基因数为6．2306；观测杂合度（He）为0．4667～0．9667；期望杂合度（H。）为0．6198～0．9318；多态信息含量（PIC）为0．5301～0．9093，表现出高的遗传多样性水平。4个群体间的遗传分化指数（Fst）在0．0132～0．0314之间，呈现出较低的遗传分化。群体间的遗传距离在0．1255～0．2458之间；通过构建UPGMA聚类树，显示日照群体和黄岛群体最先聚类，再与蓬莱群体聚类，最后与韩国群体聚类，说明中国群体与韩国群体亲缘关系较远。
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| 关 键 词： | 魁蚶 微卫星 地理群体 遗传多样性 |
| 收稿时间： | 2012/5/2 0:00:00 |
| 修稿时间： | 2012/7/24 0:00:00 |
Microsatellite analysis of genetic diversity in four geographic populations of Scapharca broughtonii |
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| Authors: | TIAN Ji-teng^ LIU Zhi-hong^ YANG Ai-guo^ WU Biao^ ZHOU Li-qing |
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| Institution: | 1 (1 Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071) (2 College of Fisheries and Life Sciences, Shanghai Ocean University, 201306) |
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| Abstract: | Genetic diversity in four geographic populations of Scapharca broughtonii from Rizhao, Huangdao, Penglai, and Korea was analyzed using twenty SSR with high polymor phism obtained in our laboratory. Results showed that the number of alleles obtained from dif ferent populations ranged in 3-17. The average number of alleles and effective alleles were 8.35 and 6. 230 6, respectively. The observed heterozygosity（Ho） and the expected heterozygosity （H,） ranged in 0. 466 7-0. 966 7 and 0. 619 8-0. 931 8, respectively. The polymorphism informa tion content（PIC） ranged in 0. 530 1-0. 909 3, indicating a high level of genetic diversity in these populations. The genetic differentiation index （Fs,） of the four geographic populations ranged in 0. 013 2-0. 031 4, revealing a low level of genetic differentiation among them. The ge netic distances among populations ranged in 0. 125 5-0. 245 8. The UPGMA tree indicated thatRizhao and Huangdao populations clustered first, then clustered with Penglai population, and further clustered with the Korean population, indicating that the Korean population was the far thest relative of the Chinese populations. The information of population structure obtained in this work would be helpful for the genetic breeding and conservation of S. broughtonii. |
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| Keywords: | Scapharca broughtonii Microsatellite Geographic populationsGenetic diversity |
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