| 微卫星分子标记指导镜鲤群体选育 |
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| 引用本文： | 鲁翠云,金万昆,李超,孙效文,杨建新.微卫星分子标记指导镜鲤群体选育[J].水产学杂志,2011,24(3):29-34,47. |
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| 作者姓名： | 鲁翠云 金万昆 李超 孙效文 杨建新 |
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| 作者单位： | 1. 中国水产科学研究院黑龙江水产研究所,黑龙江哈尔滨,150070
2. 国家级天津市换新水产良种场,天津,301500 |
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| 基金项目： | 国家重大基础研究计划资助项目，农业部“引进国际先进农业科学技术”计划，黑龙江水产研究所基本科研业务费专项资金 |
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| 摘 要： | 本文用26个扩增效果好的微卫星分子标记分析了镜鲤（Cyprinus carpio L.）养殖亲本群体的遗传结构,指导其群体选育。本研究共检测到153个等位基因,片段大小为108~400 bp,平均等位基因数（A）5.8846个,平均有效等位基因数（Ne）2.8625个,平均观察杂合度（Ho）0.5063,平均期望杂合度...
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| 关 键 词： | 微卫星 镜鲤 群体选育 |
Population Selection in Mirror Carp Cyprinus carpio L.Assisted by Microsatellite Markers |
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| Abstract: | In this paper,genetic structure of the broodstock of mirror carp（Cyprinus carpio L.） including 208 samples（106 females and 102 males） was analyzed using 26 microsatellite markers.Total 153 alleles ranging from 108 bp to 400 bp in size were screened and several genetic parameters were used to evaluate the parental population.There were 5.8846 alleles on average with the mean effective alleles of 2.8625.The mean observed heterozygosity value was found to be 0.5063 and the mean expected heterozygosity value of 0.5602.The mean value of polymorphism information content was 0.5292 in 26 microsatellite loci.The relatively high level of genetic diversity was statistically found in the breeding population.Although the population accorded with Hardy-Weinberg equilibrium by checking by χ2 test and genetic departure index,severe heterozygote deficit was shown to be derived from artificial selection in many loci.The genetic distance was varied from 0.0256 to 0.9943 among the individuals.A cluster gram was constructed based on UPGMA methods using PHYLIP 3.6 software package.Based on the map,two breeding groups were selected to breed offspring and the fry from the control population was reared from the total broodstock.The selective offspring populations showed relatively high genetic diversity with the average expected heterozygosity ranging from 0.5414 to 0.5449.In Addition,the selective offspring population had good growth,from 14.81% to 63.71% higher than that in the control population.The successive two year growth revealed that the microsatellite markers-assisted population selection could hold genetic diversity and avoid inbreeding.It is superior to construct a good growth population of the mirror carp. |
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| Keywords: | microsatellite marker mirror carp（Cyprinus carpio L ） population selection |
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