| 静水压休克法诱导三倍体鲶鱼（silurus asotus L）的研究 |
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| 引用本文： | 尹洪滨,孙中武,等.静水压休克法诱导三倍体鲶鱼（silurus asotus L）的研究[J].水产学杂志,1997,10(1):10-13. |
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| 作者姓名： | 尹洪滨 孙中武 |
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| 作者单位： | [1]中国水产科学研究院黑龙江水产研究所，哈尔滨150070 [2]东北林业大学野生动物资源学院，哈尔滨150040 |
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| 摘 要： | 采用静水压休克法诱导三倍体鲶鱼。通过对受精时间、静水压力及持续施压处理时间三方面进行筛选试验的结果表明，鲶鱼卵受精4－5min，用600－649kg/cm^2的静水压力处理3min，可以获得100％的三倍体鲶鱼，而且胚胎存活率也较高，孵化率达对照组的90％以上，是静水压休克法诱导三倍体鲶鱼的最佳条件。三倍体鲶鱼的倍性用细胞遗传学方法验证。
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| 关 键 词： | 静水压休克 鲶鱼 三倍体 人工诱导 受精时间 静水压力 持续施压处理时间 |
STUDY ON TRIPLOID OF CATFISH (Silurus asotus L.)INDUCED BY HYDROSTATIC PRESSURE SHOCK |
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| Yin Hongbin,Pan weizhi, Sun zhongwu and Qiu Lingquan.STUDY ON TRIPLOID OF CATFISH (Silurus asotus L.)INDUCED BY HYDROSTATIC PRESSURE SHOCK[J].Chinese Journal of Fisheries,1997,10(1):10-13. |
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| Authors: | Yin Hongbin Pan weizhi Sun zhongwu and Qiu Lingquan |
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| Abstract: | Hydrostatic pressure shock was used to induce and produce triploid silurus asotus L. By a series of tests for the fertilized times,hydrostatic pressure and treated times of catfish eggs, the results indicated that the best condition of inducement triploid is 4-5 min after fertilization, and that the eggs were treated for 3min at 600~640kg/cm2 of hydrostatic pressure. Which not only led to 100% of triploid Silurus asotus L. but also resulted in relatively high survival percentage of embryos. The percentage of hatching was as 90% as that of the control. The triploidy of these fish was verified by cytogenetic methods. |
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| Keywords: | hydrostatic pressure shock catfish triploid |
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