Studies on sperm of diploid and triploid tench, Tinca tinca (L.) |
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| Authors: | Otomar Linhart Marek Rodina Martin Flajshans Nicola Mavrodiev Jana Nebesarova David Gela Martin Kocour |
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| Institution: | (1) Joint Laboratory of Genetics, Physiology and Reproduction of Fish, University of South Bohemia, Research Institute of Fish Culture and Hydrobiology, 38925 Vodnany, Czech Republic;(2) Faculty of Agriculture, Department of Fisheries, University of South Bohemia, Studentska 13, 370 05 Ceske Budejovice, Czech Republic;(3) Institute of Parasitology, Academy of Sciences of the Czech Republic, Laboratory of Electron Microscopy, Branisovska 31, 370 05 Ceske Budejovice, Czech Republic |
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| Abstract: | The tench Tinca tinca is an interesting fish from the viewpoint of polyploidy and related atypical reproduction aspects. Triploid tench were produced
artificially. Studies of spermiation as well as of sperm motility and structure were performed on several triploid and diploid
males simultaneously with individual experimental crosses with diploid females to define their reproductive capacities. The
testes of triploids visually looked less developed in the most of cases with lower sperm production (0.05 cm3 sperm per male), GSI and weight of testes compared to diploids (0.58 cm3 sperm per male). Analysis of variance showed significant influence of ploidy level on the percentage of motile spermatozoa.
Triploidy did not change percentage of live spermatozoa and velocity of spermatozoa at the first time of sperm movement. The
study of sperm structure by scanning electron microscopy revealed that most sperm cells were of normal structure with some
anomalies. Sperm heads of triploid and diploid males were mostly round-shaped, 1.86±0.2 and 1.6±0.18 μm in diameter. The midpiece
of triploid spermatozoa was slightly narrower than that of diploid ones with typical cylindrical shape. Flow cytometry revealed
sperm cells of triploids to be largely aneuploid (1.47 n) with high mosaic DNA, oscillating from haploid DNA content (1.0 n)
to diploid DNA content (1.9 n). Experimental crosses between triploid males and diploid females revealed that these males
were capable to stimulate effective development with relatively high level of fertilization and hatching rates from 0 to 70%.
In conclusion, triploidization does not seem to guarantee sterility of tench. |
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| Keywords: | Aquaculture Diploid Fish biology Fish sterilization Spermatozoa Tench Tinca tinca Triploid |
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