|
|||||
|
|
Evaluation of a Fast Method Based on the Presence of Two Restriction Sites in the Mitochondrial ND5 (mt ND5) Gene for the Identification of Scomber Species |
|
| Authors: | Iciar Martinez Amaya Velasco Ricardo Pérez-Martín Eskil Forås Michiaki Yamashita Carmen G Sotelo |
| Institution: | 1. Institute of Marine Research (IIM-CSIC) , Vigo , Spain;2. Norwegian College of Fishery Science , University of Troms? , Troms? , Norway miciar_martinez@ehu.es;4. Institute of Marine Research (IIM-CSIC) , Vigo , Spain;5. SINTEF Fisheries and Aquaculture , SINTEF Sealab , Trondheim , Norway;6. National Research Institute of Fisheries Science , Yokohama , Japan |
| Abstract: | The purpose of this work was to evaluate the suitability of a method based on the presence of two restriction sites (for Hae III and Hindf I) in the mitochondrial NADH dehydrogenase subunit 5 (mt ND5) gene to identify Scomber species. The evaluation was performed on 144 reference and market samples by sequencing of the entire 505-bp fragment of the mt ND5 gene and of a 464-bp fragment of the Kocher fragment of the cytochrome b gene (mt Cytb). Sequence analysis of any of the two fragments allows the identification of each of the four Scomber species, but S. japonicus and S. colias had the same restriction sites at the ND5 amplicon and would not have been differentiated by this analysis. Similarly, loss of the Hae III site in some S. scombrus individuals would have misidentified them as not being Scomber. All the market products were correctly labeled except one acquired in Spain labeled as originating in the Atlantic and containing S. japonicus. |
| Keywords: | species identification fish mackerel Scomber PCR FINS mt DNA RFLP-PCR saba caballa scombrus colias japonicus australasicus traceability |