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鳗弧菌注射对栉孔扇贝免疫活性的影响
引用本文:樊甄姣,杨爱国,吕振明,刘志鸿.鳗弧菌注射对栉孔扇贝免疫活性的影响[J].南方水产,2007,3(6):52-55.
作者姓名:樊甄姣  杨爱国  吕振明  刘志鸿
作者单位:1. 浙江海洋学院海科学院,浙江,舟山,316000
2. 中国水产科学研究院黄海水产研究所,山东,青岛,266071
基金项目:国家高技术研究发展计划(863计划)项目(2003AA603022)
摘    要:测定了鳗弧菌(Vibrio anguillarum)注射对栉孔扇贝(Chlamys farreri)胞内活性氧(ROS)含量和过氧化氢酶(CAT)、酸性磷酸酶(ACP)和碱性磷酸酶(ALP)活性的影响。结果表明,在注射后5、24、48和72h各免疫指标都有明显变化;胞内ROS含量在24、48和72h明显升高,且均高于对照组,形成抛物线趋势,在48h达到最高;血清中CAT活性均有升高趋势;肝脏中ACP活性在24、48和72h与注射生理盐水组相比明显升高;栉孔扇贝体内ALP活性有明显升高的趋势,且在注射后24h活性达到最高,而后有所下降。结果表明,鳗弧菌对栉孔扇贝免疫系统有明显的刺激作用。

关 键 词:栉孔扇贝  鳗弧菌  活性氧  酶活性
文章编号:1673-2227-(2007)06-0052-04
修稿时间:2007年6月14日

Effects of Vibrio anguillarum on immune activities of Chlamys farreri
FAN Zhenjiao,YANG Aiguo,LV Zhenming,LIU Zhihong.Effects of Vibrio anguillarum on immune activities of Chlamys farreri[J].South China Fisheries Science,2007,3(6):52-55.
Authors:FAN Zhenjiao  YANG Aiguo  LV Zhenming  LIU Zhihong
Abstract:The content of intracellular ROS and the activities of CAT, ACP and ALP of Chlamys farreri were studied at 5, 24, 48 and 72 h after challenged with Vibrio anguillarum injection. The results showed that the intracellular ROS content of experimental groups were much higher than that of control groups at 24, 48, 72 h. The CAT activities of experimental groups in haemocytes were higher than that of control group but not significantly. The ACP and ALP activities of experimental groups were much higher than that of control group at 5, 24, 48, 72 h. In conclusion, V.anguillarum could influence the immune activities of C.farreri.
Keywords:Chlamys farreri  Vibrio anguillarum  ROS  enzyme activity
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