| 鳀科鱼类线粒体全基因组序列结构特征及系统发育信息分析 |
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| 引用本文： | 张丽丽,程起群.鳀科鱼类线粒体全基因组序列结构特征及系统发育信息分析[J].海洋渔业,2012,34(1):7-14. |
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| 作者姓名： | 张丽丽 程起群 |
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| 作者单位： | 1. 农业部海洋与河口渔业资源及生态重点开放实验室,中国水产科学研究院东海水产研究所,上海 200090;南京农业大学无锡渔业学院,江苏无锡 214081
2. 农业部海洋与河口渔业资源及生态重点开放实验室,中国水产科学研究院东海水产研究所,上海 200090 |
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| 基金项目： | 上海市科委创新行动计划项目资助 |
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| 摘 要： | 为了解鳀科鱼类的线粒体全基因组序列结构特征及系统发育信息,以期为进化遗传学研究和分子标记的选取提供参考依据,对已知的10种鳀科鱼类的线粒体全基因组进行分析。结果显示:1)鳀科线粒体基因组全序列长度在16 660 bp到17 069 bp之间,基因组的结构和基因排列顺序与其它硬骨鱼类一致。2)比对后获得一致序列长度为15 704 bp(不含D-loop),其中变异位点5 570个,占所有位点数的35.5%。在编码基因中,序列变异程度和Kimura双参数遗传距离最大的是ND6基因(分别是47.5%和0.276),最小的是tRNA拼接序列(分别为18.7%和0.072)。3)基于Ka-Ks的Z检验和Tajima’s D检验表明蛋白质编码基因主要受到净化选择(即负选择,purifying selection)的作用;其中12个蛋白质编码基因(ND6除外)有强烈的净化选择(Ka/Ks<1),而ND6基因受正选择(positive selection)影响较大(Ka/Ks>1)。4)ND4、ND2和Cytb是进行鳀科鱼类系统发育分析的较为理想的分子标记。
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| 关 键 词： | 鳀科 线粒体 基因组特征 自然选择 系统进化 |
Mitochondrial genome characteristics and phylogenetic information of family Engraulidae ( Clupeiformes: Clupeoidei) fishes |
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| ZHANG Li-li
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CHENG Qi-qun.Mitochondrial genome characteristics and phylogenetic information of family Engraulidae ( Clupeiformes: Clupeoidei) fishes[J].Marine Fisheries,2012,34(1):7-14. |
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| Authors: | ZHANG Li-li CHENG Qi-qun |
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| Institution: | 1(1.Key and Open Laboratory of Marine and Estuarine Fisheries Resources and Ecology,Ministry of Agriculture, East China Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Shanghai 200090,China; 2.Wuxi Fisheries College,Nanjing Agricultural University,Wuxi 214081,China) |
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| Abstract: | In order to understand the mitochondrial genome characteristics and phylogenetic information of family Engraulidae fishes,benefiting the study of evolutionary genetics and selection of molecular markers,all known 10 mitochondrial genomes of Engraulidae fishes were analyzed.The results were as follows: 1) Lengths of mitochondrial genomes were from 16 660bp to 17 069bp.Mitochondrial genome composition and gene order was consistent with other teleost fishes;2) After alignment,15 704 base pairs of consensus sequences were obtained,in which 5 570 were variable sites,accounting for 35.5% of total sites.Among all genes,the ND6 had the highest sequence divergence(47.5%) and kimura-2-Parameter genetic distance(0.276),whereas the concatenated tRNA had the lowest sequence divergence(18.7%) and K-2-P distance(0.072);3) Protein coding genes were mainly acted by purifying selection which was detected by Z test and Tajima’s D test.Most of the genes,except ND6,were influenced by purifying selection(Ka/Ks<1);4) ND4,ND2 and Cytb genes were suitable molecular markers for phylogenetic study of Engraulidae fishes. |
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| Keywords: | Engraulidae mitochondrial genome characteristics natural selection phylogeny |
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