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银鲳精子的超微结构
引用本文:赵峰,庄平,章龙珍,施兆鸿,黄晓荣,闫文罡.银鲳精子的超微结构[J].海洋渔业,2010,32(4).
作者姓名:赵峰  庄平  章龙珍  施兆鸿  黄晓荣  闫文罡
基金项目:中央级公益性科研院所基本科研业务费专项资金(中国水产科学研究院东海水产研究所,上海市农委重点项目
摘    要:运用扫描与透射电镜对银鲳精子的超微结构进行了观察。银鲳精子由头部、中段和尾部(鞭毛)3部分组成。精子全长约39.51±1.64μm,头部长约1.98±0.30μm,表面粗糙。头部的主要结构包括细胞核和中心粒复合体。细胞核位于头部腹侧,卵圆形。细胞核中染色质致密,存在着不规则的网络状间隙。近端中心粒为9组三联微管结构,与远端中心粒相互垂直。中段的主要结构为袖套和线粒体。袖套与细胞核后端相连,含有4~5个线粒体及少量囊泡。尾部细长,长约37.52±1.68μm,至末端逐渐变细。尾部的主要结构为轴丝,为典型的"9+2"微管结构。尾部具有波纹状侧鳍,排列不对称。

关 键 词:精子  银鲳  扫描电镜  透射电镜  超微结构

Ultrastructure of the spermatozoon in Pampus argenteus
ZHAO Feng,ZHUANG Ping,ZHANG Long-zhen,SHI Zhao-hong,HUANG Xiao-rong,YAN Wen-gang.Ultrastructure of the spermatozoon in Pampus argenteus[J].Marine Fisheries,2010,32(4).
Authors:ZHAO Feng  ZHUANG Ping  ZHANG Long-zhen  SHI Zhao-hong  HUANG Xiao-rong  YAN Wen-gang
Abstract:Ultrastructure of Pampus argenteus spermatozoa was investigated by means of scanning electron microscopy(SEM) and transmission electron microscopy(TEM).Spermatozoa of P.argenteus consisted of a head,a midpiece and a tail(flagellum).The spermatozoa,39.51±1.64 μm in total length,was asymmetrically shaped.The head,with a coarse surface,was 1.98±0.30 μm in total length.Main structure of the head was nucleus and centriolar complex.The nucleus,of spherical shape,was located at the ventral part of the head.The nucleus consisted of electron dense chromatin materials and irregularly netlike gap.The proximal centriole,which is made up of nine triplets of peripheral microtubules,and the distal cintriole were arranged at a right angle to each other.The midpiece of spermatozoa includes sleeve and mitochondria.The sleeve,which contained 4-5 mitochondria and a few vesicles,was connected to the posterior end of the nucleus.Tail was slim and long,37.52±1.68 μm in total length.The end part of the tail was much thinner than the anterior part.The main structure of the tail was axoneme,of typical "9+2" model.The tail had lateral fins,which were undulating and arranged asymmetrically.
Keywords:Spermatozoon  Pampus argenteus  scanning electron microscopy(SEM)  transmission electron microscopy(TEM)  ultrastructure
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