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| 超低温冷冻对斑尾刺虾虎鱼卵中酶活性的影响 | |
| 引用本文： | 黄晓荣,章龙珍,庄平,江琪,姚志峰,刘鉴毅.超低温冷冻对斑尾刺虾虎鱼卵中酶活性的影响[J].海洋渔业,2010,32(1):48-53. |
| 作者姓名： | 黄晓荣 章龙珍 庄平 江琪 姚志峰 刘鉴毅 |
| 作者单位： | 1. 中国水产科学研究院东海水产研究所,上海,200090;上海海洋大学水产与生命学院,上海,201306 2. 中国水产科学研究院东海水产研究所,上海,200090;浙江省杭州千岛湖鲟龙科技开发有限公司,杭州 311700 3. 中国水产科学研究院东海水产研究所,上海,200090 |
| 基金项目： | 中央级公益性科研院所基本科研业务费专项资金项目(2007T02);;上海市高校水产养殖学E-研究院建设项目(E03009);;上海市长江口中华鲟自然保护区资助项目 |
| 摘 要： | 研究了超低温冷冻保存(-196℃)对斑尾刺虾虎鱼成熟卵中总ATP酶、肌酸激酶(CK)、琥珀酸脱氢酶(SDH)、乳酸脱氢酶(LDH)、超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GSH-PX)和丙二醛(MDA)等酶活性的影响。结果表明,经过冷冻保存后,总ATP酶、CK、SDH和LDH的活性显著下降(P<0.05),同时可见添加抗冻剂组比未添加抗冻剂组下降幅度更大(P<0.05);SOD、CAT和GSH-PX的活性也显著下降(P<0.05),未添加抗冻剂组比添加抗冻剂组下降更明显(P<0.05);MDA的活性显著升高,且未添加抗冻剂组显著高于添加抗冻剂组(P<0.05)。超低温冷冻和是否添加抗冻剂都对斑尾刺虾虎鱼卵中酶活性有显著性影响。 |
| 关 键 词： | 超低温冷冻 斑尾刺虾虎鱼 卵 酶活性 |
Effects of cryopreservation on enzyme activity in Acanthogobius ommaturus ovum |
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| HUANG Xiao-rong,ZHANG Long-zhen,ZHUANG Ping,JIANG Qi,YAO Zhi-feng,LIU Jian-yi.Effects of cryopreservation on enzyme activity in Acanthogobius ommaturus ovum[J].Marine Fisheries,2010,32(1):48-53. | |
| Authors: | HUANG Xiao-rong ZHANG Long-zhen ZHUANG Ping JIANG Qi YAO Zhi-feng LIU Jian-yi |
| Institution: | 1.East China Sea Fisheries Research Institute;Chinese Academy of Fishery Sciences;Shanghai 200090;China;2.College of Fisheries and Life Science;Shanghai Ocean University;Shanghai 201306;3.Hangzhou Qiandaohu Xunlong Technology Development Co.Ltd;Hangzhou 311700;China |
| Abstract: | The effects of cryopreservation(-196 ℃) on the enzyme activity (total ATPase, CK, SDH, LDH, SOD, CAT, GSH-PX and MDA) in Acanthogobius ommaturus ovum were analysed by reagent box. The results showed that the activity of total ATP, CK, SDH and LDH decreased significantly after cryopreservation (P<0.05), but the group added cryoprotectant dropped more than the group without cryoprotectant; the activity of SOD,CAT and GSH-PX dropped significantly after cryopreservation too (P<0.05), but the group without cryoprotectant declined more than the one added cryoprotectant. The activity of MDA increased significantly, and the activity of the group without cryoprotectant was much better than that of the added group (P<0.05).Cryopreservation and cryoprotectant had great effects on the enzyme activity in Acanthogobius ommaturus ovum. |
| Keywords: | Cryopreservation Acanthogobius ommaturus ovum enzyme activity |
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