| 河川沙塘鳢3个不同地理群体杂交F_1代生长性能及遗传多样性 |
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| 引用本文： | 王佩佩,丁严冬,于兴达,贾秀琪,张国松,尹绍武,陈树桥,周国勤.河川沙塘鳢3个不同地理群体杂交F_1代生长性能及遗传多样性[J].水产科学,2016(5):528-534. |
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| 作者姓名： | 王佩佩 丁严冬 于兴达 贾秀琪 张国松 尹绍武 陈树桥 周国勤 |
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| 作者单位： | 1. 南京师范大学生命科学学院，江苏省生物多样性与生物技术重点实验室，江苏南京210023;2. 南京市水产科学研究所,江苏南京,210036 |
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| 基金项目： | 江苏省科技支撑计划(农业)项目(BE2013441)；江苏省六大人才高峰＂高层次人才项目(2012-NY-032)；江苏省2015年度普通高校研究生实践创新计划项目(SJLX15＿0300)；南京师范大学科技成果转化基金资助项目(2013-02)． |
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| 摘 要： | 选取来自浙江建德、安徽当涂、江苏太湖3个不同地理群体的河川沙塘鳢,采用3×3完全双列杂交方式进行配组,对9个组合F1子代的生长性状及遗传多样性进行比较分析。通过对2月龄和4月龄F1子代的生长性状进行对比分析发现,建德(♂)×当涂(♀)杂交组合的全长增加率、质量增加率分别为42.58%、190.79%,建德×当涂(正反交)组合的全长杂交优势率、体质量杂交优势率分别为17.88%、66.75%,均高于其他组合。不同地理群体间的杂交F1子代的全长增加率、质量增加率、杂交优势率均高于同一地理群体间的自繁F1子代。采用8个微卫星引物分析获得9个组合F1子代的遗传分化指数平均值为0.195,表明9个组合子代间存在遗传差异与分化,其期望杂合度为0.1493~0.7971,观测杂合度为0.1562~1.0000。双列杂交子代的杂合度和遗传多样性高于自繁子代,其中建德(♂)×当涂(♀)组合的等位基因数、多态信息含量值分别为3.9和0.486,均高于其他组合。综合生长性能和遗传多样性,初步选择建德(♂)×当涂(♀)为最佳杂交组合。
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| 关 键 词： | 河川沙塘鳢 地理群体 双列杂交 杂交优势 遗传多样性 |
Growth Trait and Genetic Diversity in the F1 Progeny of Dark Sleeper Odontobutis potamophila from Three Different Populations |
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| Abstract: | We chosed three different populations of dark sleeper Odontobutis potamop hila from Jiande , Dangtu and Taihu and mated by using 3 × 3 full diallel crossing .The growth traits and genetic diversity about the F1 offspring of nine combinations were compared and F1 progeny′s growth traits were evaluated in 2 months and 4 months .The length growth rate and the weight gain rate of the Jiande (♂) × Dangtu (♀) combination were shown to be 42 .58% and 190 .79% .The overall length heterosis ratio and the weight heterosis ratio of Jiande × Dangtu (cross and reciprocal cross ) combination were 17 .88% and 66 .75% ,higher than those in other combinations .There were higer length growth rate ,the weight gain rate and the heterosis rate in F1 progeny from different geographic populations than those in the same geo‐graphical population of the self‐reproducing F1 offspring .Subsequently ,the average value (0 .195) of hy‐brid progeny genetic differentiation index (Fst) was figured out by using eight microsatellite loci ,indica‐ting that there were genetic differences and the differentiation of nine combinations existed in offspring . The nine combinations of expected heterozygosity (He) were ranged from 0 .1493 to 0 .7971 and the ob‐served heterozygosity (Ho) ranged from 0 .1562 to 1 .0000 .The heterozygosity and the genetic diversity of hybrid progeny were higher than those of self‐reproducing progeny and the alleles (A) .The polymorphism information content (PIC) value of Jiande (♂) × Dangtu (♀) combination were 3 .9 and 0 .486 ,higher than other combinations .To synthesize the growth performance and the genetic diversity ,we suggest that determination of grow th and genetic diversity of the Jiande (♂) × Dangtu (♀ ) be the preliminary best combination of cross breeding . |
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| Keywords: | Odontobutis potamophila geographical group diallel cross heterosis genetic diversity |
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