| 循环海水养殖系统中生物膜快速形成的模拟试验 |
| |
| 引用本文： | 王硕,刘万鑫,杜荣斌,由翠荣,付学军,梁丽琨.循环海水养殖系统中生物膜快速形成的模拟试验[J].水产科学,2016(3):244-249. |
| |
| 作者姓名： | 王硕 刘万鑫 杜荣斌 由翠荣 付学军 梁丽琨 |
| |
| 作者单位： | 1. 烟台大学生命科学学院,山东烟台,264005;2. 烟台大学海洋学院,山东烟台,264005 |
| |
| 基金项目： | 山东省现代农业产业技术体系产业创新团队项目(SDAIT-14-011-02)；山东省科技发展计划项目(2013GNC11037，2013YD15011)． |
| |
| 摘 要： | 在20L水箱中加入15L人工污水模拟工厂化循环水养殖系统的生物滤池,采用优势菌种挂膜法,对滤材种类、混合菌液添加比例及接种次数等条件进行了优化,确定了快速挂膜的最佳实验室工作条件。在21d内对试验水体中的NH+4、NO-3和NO-2进行了监测。试验结果显示,最优试验条件为使用塑料弹性滤材作为生物膜载体,每7d接种1次优势菌液,硝化细菌∶反硝化细菌∶芽孢菌∶动胶菌添加比例为2∶1∶1∶1,在此条件下,第7d开始形成生物膜,第14d生物膜基本形成,第21d形成均匀完整的生物膜。成熟的生物膜在15d内对水体NH+4的最大降解量可超过5.00mg/L,即成熟生物膜降解NH+4的速率为每日降解超过0.50mg/L。当初始NH+4为5.00~10.00mg/L,成熟生物膜对NH+4和NO-2的降解能力在15d内可达到上限。
|
| 关 键 词： | 生物膜 快速形成 循环海水养殖 |
Simulation Test of Rapid Biofilm Formation in a Recycling Aquaculture System in a Lab |
| |
| Abstract: | Fifteen liters of artificial wastewater were added into a 20 L tank to imitate recirculating aqua‐culture system ,and laboratory conditions of rapid biofilm formation were optimized including biofilm carri‐er types ,inoculation proportion and frequency of the mixed functional bacteria .T he concentrations of NH4+ ,NO3- and NO2- in the experimental water were monitored during the rapid biofilm formation .The re‐sults showed that the optimal rapid biofilm formation was found under the conditions of elastic filter as bio‐film carrier ,and feeding dominant bacteria per 7 days with the mixing ratio of nitrobacteria :denitrifying bacteria∶ Bacillus∶ Zoogleae itzigsohn =2∶1∶1∶1 .Under these conditions ,the biofilm was observed on the seventh day ,almost complete on the fourteenth day and mature on the twenty‐first day .The purif‐ying efficiency trial indicated that the maximum removal of NH +4 by mature biofilm was over 5 .00 mg/L within 15 days ,with the maximal NH4-degradation rate of over 0 .50 mg/L per day .The maximal removal of NH+4 and NO2- was found within 15 days at initial NH4+ concentration from 5 .00 mg /L to 10 .00 mg/L . |
| |
| Keywords: | biofilm rapid formation recirculating aquaculture |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
