| 不同盐度对生物絮团、对虾生长以及酶活性的影响 |
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| 引用本文： | 秦海鹏,杨世平,王博,廖栩峥,胡世康,赵吉臣,何子豪,孙成波.不同盐度对生物絮团、对虾生长以及酶活性的影响[J].水产科学,2020,39(3):400-406. |
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| 作者姓名： | 秦海鹏 杨世平 王博 廖栩峥 胡世康 赵吉臣 何子豪 孙成波 |
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| 作者单位： | 广东海洋大学 水产学院,广东湛江 524088;广东海洋大学 水产学院,广东湛江 524088;广东海洋大学 水产学院,广东湛江 524088;广东海洋大学 水产学院,广东湛江 524088;广东海洋大学 水产学院,广东湛江 524088;广东海洋大学 水产学院,广东湛江 524088;广东海洋大学 水产学院,广东湛江 524088;广东海洋大学 水产学院,广东湛江 524088 |
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| 基金项目： | 广东省海洋渔业科技与发展专项科技攻关与研发项目(A201508B05);广东省科技计划项目(20130208c). |
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| 摘 要： | 在不同盐度条件下进行凡纳滨对虾的生物絮团养殖试验,研究盐度对生物絮团养殖水质和对虾生长及其酶活性的影响。试验设5个盐度梯度(10、15、20、25、30),生物絮团初始量为20 mL/L,对虾密度为500尾/m^3,试验周期30 d。试验结果显示,15盐度组与20盐度组的对虾体质量增长率最大,达70.73%,10盐度组的体质量增长率最小,达50.24%。盐度越高生物絮团生长越快,30盐度组17 d生物絮团沉降量达200 mL/L,之后逐渐降至43 mL/L,其他组呈相同变化趋势。试验过程中水体总碱度与pH持续降低,但不同组间差异不显著(P>0.05)。盐度越高氨氮累积越快,30盐度组在第6 d达到最大质量浓度8.62 mg/L,之后降至0 mg/L,其他组呈相同趋势变化。盐度越低亚硝态氮累积越快,10盐度组在第6 d达到最大质量浓度9.18 mg/L,之后降至0 mg/L,其他组呈相同趋势变化。硝态氮在不同盐度中呈前期上升的趋势,第16 d之后开始缓慢下降。15盐度组的淀粉酶活性显著高于其他组(P<0.05),其他各组之间无显著差异(P>0.05)。脂肪酶在25盐度组活性最高,盐度升高或者降低酶活性均降低。在10、15、20盐度组中,超氧化物歧化酶、碱性磷酸酶、酸性磷酸酶活性均维持在较高水平,在相同盐度下,肌肉酶活性低于肝胰脏。
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| 关 键 词： | 盐度 生物絮团 水质 生长 酶活性 |
Effect of Salinity on Biofloc Culture,Growth and Enzyme Activity in Pacific White Shrimp Litopenaeus vannamei |
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| QIN Haipeng,YANG Shiping,WANG Bo,LIAO Xuzheng,HU Shikang,ZHAO Jichen,HE Zihao,SUN Chengbo.Effect of Salinity on Biofloc Culture,Growth and Enzyme Activity in Pacific White Shrimp Litopenaeus vannamei[J].Fisheries Science,2020,39(3):400-406. |
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| Authors: | QIN Haipeng YANG Shiping WANG Bo LIAO Xuzheng HU Shikang ZHAO Jichen HE Zihao SUN Chengbo |
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| Institution: | (Fisheries College, Guangdong Ocean University, Zhanjiang 524088, China) |
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| Abstract: | In this study,the biofloc culture experiment was carried out under different salinity(10,15,20,25 and 30)conditions in Pacific white shrimp Litopenaeus vennamei to study the effect of salinity on the water quality of biofloc culture and the growth and enzyme activity of the shrimp.The initial amount of biofloc was 20 mL/L,the density of prawns was 500 ind/m^3,and the experimental period was 30 d.The results showed that the maximal body weight growth rate(70.73%)was observed at salinity 15 and 20,and the minimal body weight growth rate(50.24%)was observed at salinity 10.The higher the salinity,the faster the growth of the bioflocs.The 17-day biofloc sedimentation in the salinity 30 group reached 200 mL/L,and then was gradually decreased.The same trend change was observed in the other groups.The total alkalinity and pH of the water continued to be decreased during the experiment,without significant difference among the different groups(P>0.05).Ammonia nitrogen accumulation was at the fastest rate in the salinity 30 group,with the maximum concentration of 8.62 mg/L on the 6th day,then decreased to 0 mL/L,at the salinity 10 group the rate was accumulated the slowest,and also reached the maximum concentration on the 6th day,1.71 mg/L,then decreased to 0 mg/L,and the other groups showed the same trend.The higher the salinity,the faster the ammonia nitrogen accumulation,and in the salinity 30 group the maximum concentration of 8.62 mg/L was found on the 6th day,then decreased to 0 mL/L,and the other groups showed the same trend.The lower the salinity,the faster the nitrite accumulation.In the salinity 10 group the maximum concentration of 9.18 mg/L was found on the 6th day,then decreased to 0 mL/L,and the other groups showed the same trend.Nitrate nitrogen level was increased in the early stage with different salinity,and declined slowly after the 16th day.There was significantly higher amylase activity in the salinity 15 group than that in the other groups,without difference among the other groups.The maximal lipase activity at salinity 25,and the salinity is increased or the enzyme activity is decreased.The SOD,AKP and ACP activities were maintained at a high level between 15 and 25 salinity.At the same salinity,the muscle enzyme activity was lower than that in the liver. |
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| Keywords: | salinity biofloc water quality growth enzyme activity |
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