| 点带石斑鱼与褐点石斑鱼4种同工酶的比较研究 |
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| 引用本文： | 尹绍武,黄海,廖经球,雷从改,陈国华,张本.点带石斑鱼与褐点石斑鱼4种同工酶的比较研究[J].水产科学,2007,26(9):500-503. |
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| 作者姓名： | 尹绍武 黄海 廖经球 雷从改 陈国华 张本 |
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| 作者单位： | 海南大学,海洋学院,热带生物资源教育部重点实验室,海南,海口,570228 |
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| 基金项目： | 海南省教育厅高校科学研究基金;海南大学校科研和教改项目;国家农业科技成果转化基金 |
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| 摘 要： | 采用聚丙烯酰胺凝胶垂直梯度凝胶电泳法,对点带石斑鱼与褐点石斑鱼7种组织中的4种同工酶(EST、LDH、MDH、SOD)的组织特异性进行比较研究。试验结果表明:2种石斑鱼的4种同工酶均具有明显的组织特异性。2种石斑鱼同一组织间MDH酶谱没有差别;LDH酶谱相似,只在迁移率上略有差异;EST、SOD酶谱间存在不同程度的种间差异,可作为鉴定点带石斑鱼与褐点石斑鱼的遗传标记。
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| 关 键 词： | 点带石斑鱼 褐点石斑鱼 同工酶 组织特异性 |
| 文章编号： | 1003-1111（2007）09-500-04 |
| 修稿时间： | 2006-07-17 |
Comparative Studies on Four Isozymes between Grouper Epinephelus malabaricus and E,fuscoguttatus |
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| YIN Shao-wu,HUANG Hai,LIAO Jing-qiu,LEI Cong-gai,CHEN Guo-hua,ZHANG Ben.Comparative Studies on Four Isozymes between Grouper Epinephelus malabaricus and E,fuscoguttatus[J].Fisheries Science,2007,26(9):500-503. |
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| Authors: | YIN Shao-wu HUANG Hai LIAO Jing-qiu LEI Cong-gai CHEN Guo-hua ZHANG Ben |
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| Institution: | College of Ocean, Hainan University, Key Laboratory of Tropical Biology Resources, Ministry of Education, PRC, Haikou 570228, China |
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| Abstract: | Tissue specificities of esterase(EST),superoxide dismutase(SOD),lactate dehydrogenase(LDH) and malate dehydrogenase(MDH) were analyzed in eyes,hearts,livers,kidneys,spleens,caudal fins and muscles from groupers Epinephelus malabaricus and E.fuscoguttatus by vertical polyacrylamide gel electrophoresis.The results showed that there were significant differences in tissue-specificity of the four isozymes in both groupers.For the same tissue,however,there were no significant differences in enzymatic spectra of MDH between the two groupers.There were almost no differences in enzymatic spectra of LDH between the two groupers,except for a little difference in transfer-ratio.Some differences in EST and SOD were observed between the two groupers,which can be used as the species genetic markers. |
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| Keywords: | Epinephelus malabaricus E fuscoguttatus isozyme tissue specificity |
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