| 多重PCR在创伤弧菌快速检测中的应用 |
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| 引用本文： | 张新中,张世秀,李海平,符有利,周永灿,谢珍玉.多重PCR在创伤弧菌快速检测中的应用[J].水产科学,2007,26(12):668-670. |
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| 作者姓名： | 张新中 张世秀 李海平 符有利 周永灿 谢珍玉 |
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| 作者单位： | 海南大学,海洋学院,海南,海口,570228 |
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| 基金项目： | 科技部国际科技合作项目;海南省重点实验室基金 |
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| 摘 要： | 建立多重PCR方法检测水体及海产品中的创伤弧菌。针对创伤弧菌的gyrB基因的不同位点设计了3对引物，扩增片断的大小分别为481、702、1065bp。该方法的灵敏度为10^2cfu／ml。共对3种海产品进行了创伤弧菌的检测，结果在3种海产品中均能特异性地检测到创伤弧菌。该方法快速，灵敏度高，特异性强。为海产品及水体中的创伤弧菌的检测提供了有效的方法。
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| 关 键 词： | 多重PCR 创伤弧菌 检测 |
| 文章编号： | 1003-1111(2007)12-0668-03 |
| 修稿时间： | 2006年10月30 |
The Application of Multiple PCR in Rapid Identification of Bacterium Vibro vulnificus |
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| ZHANG Xin-zhong,ZHANG Shi-xiu,LI Hai-ping,FU You-li,ZHOU Yong-can,XIE Zhen-yu.The Application of Multiple PCR in Rapid Identification of Bacterium Vibro vulnificus[J].Fisheries Science,2007,26(12):668-670. |
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| Authors: | ZHANG Xin-zhong ZHANG Shi-xiu LI Hai-ping FU You-li ZHOU Yong-can XIE Zhen-yu |
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| Abstract: | Three primers have been designed to correspond to the different sites of the gyrB gene of bacterium Vbrio vulnificus to find an approach to identify the bacteria in waters and in fishery products by a multiple polymerase chain reaction(PCR).The amplification fragments are 481 bp,702 bp and 1065 bp in length with the sensitivity of 102cfu/ml.Vibro vulnificus had been found in all the three kinds of fishery products tested,indicating that the rapid,sensitive and specific method is an efficient way to identify the bacterium in waters and in fishery products. |
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| Keywords: | multiple PCR Vibro vulnificus identification |
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