| 点带石斑鱼不同组织同工酶的研究 |
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| 引用本文： | 尹绍武,雷从改,黄海,陈国华,张本,许丽丽,黄丹,许声济.点带石斑鱼不同组织同工酶的研究[J].水产科学,2006,25(9):471-473. |
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| 作者姓名： | 尹绍武 雷从改 黄海 陈国华 张本 许丽丽 黄丹 许声济 |
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| 作者单位： | 海南大学,海洋学院,海口,570228 |
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| 摘 要： | 运用聚丙烯酰胺垂直梯度凝胶电泳法对点带石斑鱼7种组织（眼睛、肌肉、心脏、肝脏、肾脏、尾鳍、脾脏）的4种同工酶（EST、SOD、LDH、MDH）进行了初步研究，并对4种同工酶的同工酶位点及其酶谱表型进行了分析。结果表明，点带石斑鱼的4种同工酶都具有不同程度的组织特异性：EST由4个基因位点编码，具有多态现象；肌肉SOD与其它组织SOD的差异最明显；眼睛LDH与其他组织LDH的差异最明显；MDH具有线粒体型（m-MDH）和上清液型（s-MDH）两种类型。
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| 关 键 词： | 点带石斑鱼 同工酶 电泳 组织 |
| 文章编号： | 1003-1111（2006）09-0471-03 |
| 收稿时间： | 2005-08-07 |
| 修稿时间： | 2005-10-08 |
Isozymes of Different Tissues in Grouper Epinphelus malabaricus |
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| YIN Shao-wu,LEI Cong-gai,HUANG Hai,CHEN Guo-hua,ZHANG Ben,XU Li-li,HUANG Dan,XU Sheng-ji.Isozymes of Different Tissues in Grouper Epinphelus malabaricus[J].Fisheries Science,2006,25(9):471-473. |
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| Authors: | YIN Shao-wu LEI Cong-gai HUANG Hai CHEN Guo-hua ZHANG Ben XU Li-li HUANG Dan XU Sheng-ji |
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| Institution: | College of Ocean, Hainan University, Haikou 570228 ,China |
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| Abstract: | Four isozymes(esterase EST,superoxide dismutase SOD,lactate dehydrogenase LDH and malate dehydrogenase MDH) from seven tissues(eyes,muscles,heart,liver,kidney,caudal fin and spleen) of E.malabaricus were studied by vertical polyacrylamide gel gradient electrophoresis.The loci and phenotypes of the four isozymes were analyzed.The results showed that the four isozymes presented tissue specificity.EST which was encoded by four loci had strong polymorphism.SOD in muscle was most special.Most specific LDH was observed in eye.Two types of MDH were found in E.malabaricus. |
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| Keywords: | Epinphelus malabaricus isozyme electrophoresis tissue |
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