| 华南沿海长鳍篮子鱼不同地理群体的遗传多样性分析 |
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| 引用本文： | 彭 敏,肖 珊,潘传燕,冯鹏霏,李 旻,曾地刚,杨春玲,朱威霖,蒋伟明,陈秀荔.华南沿海长鳍篮子鱼不同地理群体的遗传多样性分析[J].水生态学杂志,2022,43(5):127-133. |
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| 作者姓名： | 彭 敏 肖 珊 潘传燕 冯鹏霏 李 旻 曾地刚 杨春玲 朱威霖 蒋伟明 陈秀荔 |
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| 作者单位： | 广西壮族自治区水产科学研究院,广西壮族自治区水产引育种中心,广西壮族自治区水产科学研究院,广西壮族自治区水产科学研究院,广西壮族自治区水产科学研究院,广西壮族自治区水产科学研究院,广西壮族自治区水产科学研究院,广西壮族自治区水产科学研究院,广西壮族自治区水产科学研究院,广西壮族自治区水产科学研究院 |
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| 基金项目： | 国家海水鱼体系北海综合试验站（CARS-47-z17）,广西创新驱动发展专项资金项目（桂科AA17204088-2） |
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| 摘 要： | 对南海北部沿岸及东海南部9个不同地理群体的长鳍篮子鱼(Siganus canaliculatus)进行遗传多样性及遗传结构分析。484条DNA D-loop 序列分析结果显示：序列长度约 828bp,包含57个变异位点,92个单倍型;所有群体总的单倍型多样性指数(Hd)和核苷酸多样性指数(π)分别为0.80798和0.00405;群体内的遗传距离 0.00386～0.00532,群体间的遗传距离 0.00383～0.00482,群体间的分化指数为-0.01935～0.00759,各种分组的AMOVA分析均显示群体内变异比例超过100%;Fu"s Fs检验值为显著负值(-25.53678,p<0.01),核苷酸错配分布图呈单峰,吻合度检验数值较小且不显著,这些结果均提示长鳍篮子鱼可能经历过种群扩张事件,推测扩张时间约在4.74万年～1.18万年前。综上,南海北部沿岸及东海南部9个长鳍篮子鱼群体间不存在显著的地理结构和谱系结构,可划归一个管理单元进行种质资源保护。
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| 关 键 词： | 长鳍篮子鱼 线粒体控制区 遗传多样性 遗传结构 |
| 收稿时间： | 2021/4/28 0:00:00 |
| 修稿时间： | 2022/9/9 0:00:00 |
Genetic Diversity Analysis of Different Geographical Populations of Siganus canaliculatus Along the South China Coast |
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| PENG Min,XIAO Shan,PAN Chuan-yan,FENG Peng-fei,LI Min,ZENG Di-gang,YANG Chun-ling,ZHU Wei-lin,JIANG Wei-ming,CHEN Xiu-li.Genetic Diversity Analysis of Different Geographical Populations of Siganus canaliculatus Along the South China Coast[J].Journal of Hydroecology,2022,43(5):127-133. |
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| Authors: | PENG Min XIAO Shan PAN Chuan-yan FENG Peng-fei LI Min ZENG Di-gang YANG Chun-ling ZHU Wei-lin JIANG Wei-ming CHEN Xiu-li |
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| Institution: | Guangxi Academy of Fishery Sciences,Guangxi Key Laboratory of Aquatic Genetic Breeding and Healthy Aquaculture,Nanning Guangxi,Guangxi Aquatic Species Introduction and Breeding Center,Nanning Guangxi,Guangxi Academy of Fishery Sciences,Guangxi Key Laboratory of Aquatic Genetic Breeding and Healthy Aquaculture,Nanning Guangxi,Guangxi Academy of Fishery Sciences,Guangxi Key Laboratory of Aquatic Genetic Breeding and Healthy Aquaculture,Nanning Guangxi,Guangxi Academy of Fishery Sciences,Guangxi Key Laboratory of Aquatic Genetic Breeding and Healthy Aquaculture,Nanning Guangxi,Guangxi Academy of Fishery Sciences,Guangxi Key Laboratory of Aquatic Genetic Breeding and Healthy Aquaculture,Nanning Guangxi,Guangxi Academy of Fishery Sciences,Guangxi Key Laboratory of Aquatic Genetic Breeding and Healthy Aquaculture,Nanning Guangxi,Guangxi Academy of Fishery Sciences,Guangxi Key Laboratory of Aquatic Genetic Breeding and Healthy Aquaculture,Nanning Guangxi,Guangxi Academy of Fishery Sciences,Guangxi Key Laboratory of Aquatic Genetic Breeding and Healthy Aquaculture,Nanning Guangxi,Guangxi Academy of Fishery Sciences,Guangxi Key Laboratory of Aquatic Genetic Breeding and Healthy Aquaculture,Nanning Guangxi |
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| Abstract: | The genetic diversity and genetic structure of Siganus canaliculatus(S. canaliculatus) from 9 different geographical groups in the northern coast of the South China Sea and the southern East China Sea were analyzed. The analysis results of 484 DNA D-loop sequences showed that the sequence length was about 828 bp, containing 57 mutation sites, and 92 haplotypes; the overall haplotype diversity index and nucleotide diversity index were 0.80798 and 0.00405, respectively, which exhibited the characteristics of high haplotype diversity and oligonucleotide diversity; genetic distances within the populations were 0.00386-0.00532, genetic distances between populations were 0.00383-0.00482, and differentiation indexes between populations were -0.01935~0.00759. AMOVA analysis of various groups showed that the proportion of variation within the populations exceeded 100%; the result of neutral test showed that the Fu"s Fs test value was a significant negative value (-25.53678, p<0.01); the distribution of nucleotide mismatch showed a single peak, and the coincidence test value was small and insignificant. These suggested that S. canaliculatus may have experienced a population expansion event, and the expansion time was estimated to be about 47,400 years to 11,800 years ago. In summary, there was no significant geographic structure and pedigree structure among the 9 S. canaliculatus populations on the northern coast of the South China Sea and the southern East China Sea, and they can be classified as a management unit for germplasm resource protection. |
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| Keywords: | Siganus canaliculatus mitochondrial control region genetic diversity genetic structure |
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