| 3种染色体组加倍方法诱导黄河鲇雌核发育的比较 |
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| 引用本文： | 彭新亮,李学军,程果,乔志刚,石灵.3种染色体组加倍方法诱导黄河鲇雌核发育的比较[J].水生态学杂志,2008,29(2):44-47. |
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| 作者姓名： | 彭新亮 李学军 程果 乔志刚 石灵 |
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| 作者单位： | 河南师范大学生命科学学院 河南新乡;河南师范大学生命科学学院 河南新乡;河南师范大学生命科学学院 河南新乡;河南师范大学生命科学学院 河南新乡;河南师范大学生命科学学院 河南新乡 |
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| 摘 要： | 用经紫外线照射后遗传失活的黄河鲇精子、刺激性成熟的黄河鲇卵子, 进行雌核发育, 采用冷休克、热休克和秋水仙素浸泡等3种染色体组加倍方法诱导黄河鲇卵进行二倍体雌核发育。实验结果表明: 在人工诱导黄河鲇二倍体雌核发育过程中, 以冷休克处理组的效果最好, 胚胎孵化率达到8. 1%; 其次为秋水仙素处理组, 胚胎孵化率达到6. 3%; 热休克处理组诱导效果较差, 胚胎孵化率仅为4. 0%。
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| 关 键 词： | 黄河鲇 二倍体雌核发育 冷休克 热休克 秋水仙素 |
The Contrastive Study on Induction of Gynogenesis by Three Different Methods of Chromosome Doubling in Silurus asotus |
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| PENG Xin-liang,LI Xue-jun,CHENG Guo,QIAO Zhi-gang and SHI Ling.The Contrastive Study on Induction of Gynogenesis by Three Different Methods of Chromosome Doubling in Silurus asotus[J].Journal of Hydroecology,2008,29(2):44-47. |
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| Authors: | PENG Xin-liang LI Xue-jun CHENG Guo QIAO Zhi-gang and SHI Ling |
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| Institution: | College of Life Science , Normal University of Henan , Xinxiang 453007 , China,College of Life Science , Normal University of Henan , Xinxiang 453007 , China,College of Life Science , Normal University of Henan , Xinxiang 453007 , China,College of Life Science , Normal University of Henan , Xinxiang 453007 , China and College of Life Science , Normal University of Henan , Xinxiang 453007 , China |
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| Abstract: | The Silurus asotus eggs were activated by UV-irradiated Silurus asotus sperm, chromosome doubling was made on the induction of gynogenesis in Silurus asotus by cold-shocking ,hot-shocking and colchicines. The study showed that optimum method for chromosome doubling in induction of gynogenesis in Silurus asotus was cold-shocking , the hatch rate may reach 8.1% , the second fine method was the induced by colchicines, and it's hatch rate was 6.3%, the hot-shocking method for chromosome doubling in induction of gynogenesis in Silurus asotus was worst, it's hatch rate only reached 4.0%. |
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| Keywords: | Silurus asotus gynogenesis of diploid cold-shocking hot-shocking colchicines |
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