| 零换水条件下饲料蛋白水平对团头鲂幼鱼生长、消化酶活力和血清生化指标的影响 |
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| 引用本文： | 孙盛明,戈贤平,朱健,张武肖,苏艳莉.零换水条件下饲料蛋白水平对团头鲂幼鱼生长、消化酶活力和血清生化指标的影响[J].水生态学杂志,2017,38(1):68-74. |
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| 作者姓名： | 孙盛明 戈贤平 朱健 张武肖 苏艳莉 |
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| 作者单位： | 中国水产科学研究院淡水渔业研究中心,中国水产科学研究院淡水渔业研究中心,中国水产科学研究院淡水渔业研究中心,南京农业大学无锡渔业学院,南京农业大学无锡渔业学院 |
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| 基金项目： | 国家大宗淡水鱼类产业技术体系华东养殖岗位(CARS-46-14)；十二五国家科技支撑计划“长江下游池塘高效生态养殖技术集成与示范”(2012BAD25B07)；中央级公益性科研院所基本科研业务费专项资金(2014A08XK01) |
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| 摘 要： | 为探究零换水养殖水体中饲料蛋白水平对团头鲂(Megalobrama amblycephala)幼鱼生长、消化酶活力和血清生化指标的影响,实验设计投喂4个不同蛋白水平的生物絮团试验组(BF-20%、BF-25%、BF-30%、BF-35%)以及1个投喂35%蛋白的循环水对照组,每个处理组设置3重复,每个养殖桶放养初始体重为(6.06±0.01)g团头鲂幼鱼25尾,养殖周期为6周。结果表明:(1)形成的生物絮团可以有效地调节水质,降低水体中的氨氮、硝酸盐氮和亚硝酸盐氮水平;(2)BF-30%和BF-35%组和对照组的终末体重、增重率和饲料系数差异不显著(P0.05),但其显著高于BF-20%组(P0.05),各处理组间的存活率没有显著性差异(P0.05);(3)BF-25%组、BF-30%组和BF-35%组的肠道、肝脏超氧化物歧化酶和谷胱甘肽过氧化物酶活性显著高于对照组和BF-20%组(P0.05),而BF-20%组的肝脏丙二醛含量显著高于其他处理组(P0.05);BF-25%组、BF-30%组和BF-35%组的血清碱性磷酸酶和溶菌酶活性显著高于对照组和BF-20%组(P0.05),而其血清中总蛋白含量、白蛋白含量、谷丙转氨酶和谷草转氨酶活力差异不显著(P0.05)。研究表明,零换水条件下饲料中35%蛋白水平减少至25%并不影响团头鲂幼鱼的生长和非特异性免疫力。
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| 关 键 词： | 团头鲂 生物絮团 蛋白水平 生长 血液生化指标 |
| 收稿时间： | 2015/11/11 0:00:00 |
| 修稿时间： | 2016/11/24 0:00:00 |
Effects of Dietary Protein Level on Growth Rate, Digestive Enzyme Activity and Blood Biochemistry of Juvenile Megalobrama amblycephala Reared in Biofloc-based Culture Tanks |
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| SUN Sheng-ming,GE Xian-ping,ZHU Jian,ZHANG Wu-xiao and SU Yan-li.Effects of Dietary Protein Level on Growth Rate, Digestive Enzyme Activity and Blood Biochemistry of Juvenile Megalobrama amblycephala Reared in Biofloc-based Culture Tanks[J].Journal of Hydroecology,2017,38(1):68-74. |
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| Authors: | SUN Sheng-ming GE Xian-ping ZHU Jian ZHANG Wu-xiao and SU Yan-li |
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| Institution: | Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences,Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences,Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences,Wuxi Fisheries College, Nanjing Agricultural University,Wuxi Fisheries College, Nanjing Agricultural University |
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| Abstract: | The biofloc technology was proposed as a sustainable solution to culture fish with low protein feeds even in intensive systems, which can effectively control water quality under negligible water exchange and sustain healthy culture of fish. This study was conducted to evaluate the effects of four dietary protein levels (BF-20%, BF-25%, BF-30% and BF-35%) and circulating water control group (35% protein level) on selected growth performance, digestive enzymes activities and serum biochemistry parameters of juveniles Megalobrama amblycephala reared in biofloc-based tanks for 6 weeks. Good water quality was maintained with the promotion and development of biofloc through glucose addition during the feeding experiment. At the end of the experiment, the growth (in terms of final weight, weight gain and specific growth rate) and feed conversion rate (FCR) of fish between BF-25%, BF-30%, BF-35% and control group showed no significant differences (P > 0.05), and no significant differences (P > 0.05) on survival rates were observed among all treatment groups. The activities of protease of intestine in BF-25%, BF-30%, BF-35% group were significantly higher than that of control group, but no significant differences were observed on the activities of amylase and lipase among all treatment groups (P > 0.05). The activities of superoxide dismutase and catalase of liver in the BF-25%, BF-30%, BF-35% group were significantly higher than that of control group, however, the malondialdehyde content was significantly lower than that of control group (P < 0.05). The fish in the BF-25% group had the lowest alkaline phosphatase (ALK) and lysozyme (LZM) activities in the serum (P < 0.05), and no significant differenceswere found in the non-sepecific immunity (ALK and LZM) of fish fed with 25%, 30% and 35% dietary protein levels (P > 0.05). No significant differences were observed on the content of total protein, albumin and activitires of aspartate transaminase, alanine transaminase among all experimental groups showed (P > 0.05). The results of this study indicated that, when juveniles of M. amblycephala were reared in biofloc-based tanks, dietary protein level can be reduced from 35% to 25% without affecting growth performance and physiological response and antioxidant capability, indicating that the promoted biofloc could contribute to the protein nutrition of cultured fish. |
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| Keywords: | Megalobrama amblycephala biofloc technology protein level growth performance blood biocheistry |
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